No evidence that crayfish carcasses produce detectable environmental DNA (eDNA) in a stream enclosure experiment

被引:22
作者
Curtis, Amanda N. [1 ]
Larson, Eric R. [1 ,2 ]
机构
[1] Univ Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA
基金
美国农业部;
关键词
Invasive species; Endangered species; Procambarus clarkii; Red swamp crayfish; Wildlife management; ORCONECTES-RUSTICUS; INVASIVE CRAYFISHES; TEMPERATURE; SURVEILLANCE; DEGRADATION; INFERENCE; LAKES; TOOL;
D O I
10.7717/peerj.9333
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Environmental DNA (eDNA) is an emerging tool for monitoring invasive and imperiled species, particularly at low densities. However, the factors that control eDNA production, transport, and persistence in aquatic systems remain poorly understood. For example, the extent to which carcasses produce detectable eDNA is unknown. If positive detections are associated with dead organisms, this could confound monitoring for imperiled or invasive species. Here, we present results from one of the first studies to examine carcass eDNA in situ by deploying carcasses of the invasive red swamp crayfish (Procambarus clarkii) in a stream enclosure experiment for 28 days. We predicted that carcasses would initially produce eDNA that would decline over time as carcasses decayed. Unsurprisingly, crayfish carcasses lost biomass over time, but at the conclusion of our experiment much of the carapace and chelae remained. However, no eDNA of P. clarkii was detected in any of our samples at the crayfish density (15 P. clarkii carcasses at similar to 615 g of biomass initially), stream flow (520-20,319 L/s), or temperature (similar to 14-25 degrees C) at our site. Subsequent analyses demonstrated that these results were not the consequence of PCR inhibition in our field samples, poor performance of the eDNA assay for intraspecific genetic diversity within P. clarkii, or due to the preservation and extraction procedure used. Therefore, our results suggest that when crayfish are relatively rare, such as in cases of new invasive populations or endangered species, carcasses may not produce detectable eDNA. In such scenarios, positive detections from field studies may be more confidently attributed to the presence of live organisms. We recommend that future studies should explore how biomass, flow, and differences in system (lentic vs. lotic) influence the ability to detect eDNA from carcasses.
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页数:21
相关论文
共 62 条
[1]   The ecology of environmental DNA and implications for conservation genetics [J].
Barnes, Matthew A. ;
Turner, Cameron R. .
CONSERVATION GENETICS, 2016, 17 (01) :1-17
[2]   Multimodel inference - understanding AIC and BIC in model selection [J].
Burnham, KP ;
Anderson, DR .
SOCIOLOGICAL METHODS & RESEARCH, 2004, 33 (02) :261-304
[3]   Using eDNA to detect the distribution and density of invasive crayfish in the Honghe-Hani rice terrace World Heritage site [J].
Cai, Wang ;
Ma, Zhuxin ;
Yang, Chunyan ;
Wang, Lin ;
Wang, Wenzhi ;
Zhao, Guigang ;
Geng, Yupeng ;
Yu, Douglas W. .
PLOS ONE, 2017, 12 (05)
[4]   Environmental DNA Sampling Informs Fish Eradication Efforts: Case Studies and Lessons Learned [J].
Carim, K. J. ;
Bean, N. J. ;
Connor, J. M. ;
Baker, W. P. ;
Jaeger, M. ;
Ruggles, M. P. ;
McKelvey, K. S. ;
Franklin, T. W. ;
Young, M. K. ;
Schwartz, M. K. .
NORTH AMERICAN JOURNAL OF FISHERIES MANAGEMENT, 2020, 40 (02) :488-508
[5]   Development and field validation of an environmental DNA (eDNA) assay for invasive clams of the genus Corbicula [J].
Cowart, Dominique A. ;
Renshaw, Mark A. ;
Gantz, Crysta A. ;
Umek, John ;
Chandra, Sudeep ;
Egan, Scott P. ;
Lodge, David M. ;
Larson, Eric R. .
MANAGEMENT OF BIOLOGICAL INVASIONS, 2018, 9 (01) :27-37
[6]   Environmental DNA (eDNA) applications for the conservation of imperiled crayfish (Decapoda: Astacidea) through monitoring of invasive species barriers and relocated populations [J].
Cowart, Dominique A. ;
Breedveld, Koert G. H. ;
Ellis, Maria J. ;
Hall, Joshua M. ;
Larson, Eric R. .
JOURNAL OF CRUSTACEAN BIOLOGY, 2018, 38 (03) :257-266
[7]   From molecules to management: Adopting DNA-based methods for monitoring biological invasions in aquatic environments [J].
Darling, John A. ;
Mahon, Andrew R. .
ENVIRONMENTAL RESEARCH, 2011, 111 (07) :978-988
[8]   Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms [J].
de Souza, Lesley S. ;
Godwin, James C. ;
Renshaw, Mark A. ;
Larson, Eric .
PLOS ONE, 2016, 11 (10)
[9]   Persistence of Environmental DNA in Freshwater Ecosystems [J].
Dejean, Tony ;
Valentini, Alice ;
Duparc, Antoine ;
Pellier-Cuit, Stephanie ;
Pompanon, Francois ;
Taberlet, Pierre ;
Miaud, Claude .
PLOS ONE, 2011, 6 (08)
[10]   Evaluation of detection probabilities at the water-filtering and initial PCR steps in environmental DNA metabarcoding using a multispecies site occupancy model [J].
Doi, Hideyuki ;
Fukaya, Keiichi ;
Oka, Shin-ichiro ;
Sato, Keiichi ;
Kondoh, Michio ;
Miya, Masaki .
SCIENTIFIC REPORTS, 2019, 9 (1)