Pathogenic and pandemic Vibrio parahaemolyticus detection in fish and shellfish isolates

被引：0

作者：

Anjay ^{[1
]}

Das, S. C. ^{[2
]}

Kumar, A. ^{[3
]}

Kaushik, P. ^{[1
]}

Kumari, Savita ^{[4
]}

机构：

[1] Bihar Vet Coll, Dept Vet Publ Hlth, Patna 800014, Bihar, India

[2] IVRI, ERS, Div Vet Publ Hlth, Kolkata 700037, W Bengal, India

[3] IVRI, Div Vet Publ Hlth, Bareilly 243122, Uttar Pradesh, India

[4] BVC, Dept Vet Microbiol, Patna 800014, Bihar, India

来源：

INDIAN JOURNAL OF GEO-MARINE SCIENCES | 2016年 / 45卷 / 09期

关键词：

Vibrio parahaemolyticus; tdh-gene; trh-gene; toxRS region; GS-; PCR; O3-K6; CLONE; OYSTERS; SEAFOOD; TRH; PREVALENCE; GENE; TDH;

D O I：

暂无

中图分类号：

P7 [海洋学];

学科分类号：

0707 ;

摘要：

In this study, one hundred and seventy isolates of V. parahaemolyticus from marine water fish and shellfish were analyzed by PCR for the presence of tdh and trh virulence genes and toxRS region pandemic gene. The tdh and trh gene was detected in 55.3% and 0.59% of isolates respectively; whereas, toxRS region was detected in 34.1% isolates. Findings of the present study suggested that marine water fish and shellfish isolates of V. parahaemolyticus harbor pathogenic as well as pandemic genes which may serve as source for clinical infection through food chain.

引用

页码：1195 / 1198

页数：4

共 22 条

[11] SEQUENCE VARIATION IN THE THERMOSTABLE DIRECT HEMOLYSIN-RELATED HEMOLYSIN (TRH) GENE OF VIBRIO-PARAHAEMOLYTICUS [J].

KISHISHITA, M ;

MATSUOKA, N ;

KUMAGAI, K ;

YAMASAKI, S ;

TAKEDA, Y ;

NISHIBUCHI, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (08) :2449-2457

[12] Pandemic spread of an O3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses [J].

Matsumoto, C ;

Okuda, J ;

Ishibashi, M ;

Iwanaga, M ;

Garg, P ;

Rammamurthy, T ;

Wong, HC ;

Depaola, A ;

Kim, YB ;

Albert, MJ ;

Nishibuchi, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (02) :578-585

[13] Outbreak of Vibrio parahaemolyticus gastroenteritis associated with Alaskan oysters [J].

McLaughlin, JB ;

DePaola, A ;

Bopp, CA ;

Martinek, KA ;

Napolilli, NP ;

Allison, CG ;

Murray, SL ;

Thompson, EC ;

Bird, MM ;

Middaugh, JP .

NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (14) :1463-1470

[14]

Ogawa H., 1989, HIROSHIMA J VET MED, V4, P47

[15]

Pal S. C., 1985, BACTERIAL DIARRHEAL, P65

[16]

Pereira Chistiane Soares, 2004, Food Sci. Technol (Campinas), V24, P591, DOI 10.1590/S0101-20612004000400019

[17] Vibrio parahaemolyticus:: A concern of seafood safety [J].

Su, Yi-Cheng ;

Liu, Chengchu .

FOOD MICROBIOLOGY, 2007, 24 (06) :549-558

[18] DETECTION OF THE THERMOSTABLE DIRECT HEMOLYSIN GENE (TDH) AND THE THERMOSTABLE DIRECT HEMOLYSIN-RELATED HEMOLYSIN GENE (TRH) OF VIBRIO-PARAHAEMOLYTICUS BY POLYMERASE CHAIN-REACTION [J].

TADA, J ;

OHASHI, T ;

NISHIMURA, N ;

SHIRASAKI, Y ;

OZAKI, H ;

FUKUSHIMA, S ;

TAKANO, J ;

NISHIBUCHI, M ;

TAKEDA, Y .

MOLECULAR AND CELLULAR PROBES, 1992, 6 (06) :477-487

[19] Detection of the tdh and trh genes in Vibrio parahaemolyticus isolates in fish and mussels from Middle Black Sea Coast of Turkey [J].

Terzi, G. ;

Buyuktanir, O. ;

Yurdusev, N. .

LETTERS IN APPLIED MICROBIOLOGY, 2009, 49 (06) :757-763

[20] Isolation of a pandemic O3:K6 clone of a Vibrio parahaemolyticus strain from environmental and clinical sources in Thailand [J].

Vuddhakul, V ;

Chowdhury, A ;

Laohaprertthisan, V ;

Pungrasamee, P ;

Patararungrong, N ;

Thianmontri, P ;

Ishibashi, M ;

Matsumoto, C ;

Nishibuchi, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (06) :2685-2689

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