A role for E-cadherin in ensuring cohesive migration of a heterogeneous population of non-epithelial cells

被引：43

作者：

Campbell, Kyra ^{[1
,2
]}

Casanova, Jordi ^{[1
,2
]}

机构：

[1] CSIC, Inst Biol Mol Barcelona, E-08028 Barcelona, Catalonia, Spain

[2] Inst Recerca Biomed Barcelona, Barcelona 08028, Catalonia, Spain

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

关键词：

DROSOPHILA EMBRYO; ADHERENS JUNCTION; DE-CADHERIN; MIDGUT; EXPRESSION; POLARITY; CELLULARIZATION; TRAFFICKING; ENDOCYTOSIS; TRANSPORT;

D O I：

10.1038/ncomms8998

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Collective cell migration is a key process underlying the morphogenesis of many organs as well as tumour invasion, which very often involves heterogeneous cell populations. Here we investigated how such populations can migrate cohesively in the Drosophila posterior midgut, comprised of epithelial and mesenchymal cells and show a novel role for the epithelial adhesion molecule E-cadherin (E-Cad) in mesenchymal cells. Despite a lack of junctions at the ultrastructure level, reducing E-Cad levels causes mesenchymal cells to detach from one another and from neighbouring epithelial cells; as a result, coordination between the two populations is lost. Moreover, Bazooka and recycling mechanisms are also required for E-Cad accumulation in mesenchymal cells. These results indicate an active role for E-Cad in mediating cohesive and ordered migration of non-epithelial cells, and discount the notion of E-Cad as just an epithelial feature that has to be switched off to enable migration of mesenchymal cells.

引用

页数：11

共 33 条

[1] Endodermal and mesenchymal cross talk: a crossroad for the maturation of foregut organs [J].

Arterbery, Adam S. ;

Bogue, Clifford W. .

PEDIATRIC RESEARCH, 2014, 75 (01) :120-126

[2]

BRAND AH, 1993, DEVELOPMENT, V118, P401

[3] Mechanical Feedback through E-Cadherin Promotes Direction Sensing during Collective Cell Migration [J].

Cai, Danfeng ;

Chen, Shann-Ching ;

Prasad, Mohit ;

He, Li ;

Wang, Xiaobo ;

Choesmel-Cadamuro, Valerie ;

Sawyer, Jessica K. ;

Danuser, Gaudenz ;

Montell, Denise J. .

CELL, 2014, 157 (05) :1146-1159

[4] Specific GATA Factors Act as Conserved Inducers of an Endodermal-EMT [J].

Campbell, Kyra ;

Whissell, Gavin ;

Franch-Marro, Xavier ;

Batlle, Eduard ;

Casanova, Jordi .

DEVELOPMENTAL CELL, 2011, 21 (06) :1051-1061

[5] Hexagonal packing of Drosophila wing epithelial cells by the planar cell polarity pathway [J].

Classen, AK ;

Anderson, KI ;

Marois, E ;

Eaton, S .

DEVELOPMENTAL CELL, 2005, 9 (06) :805-817

[6] NEW INSIGHTS INTO THE REGULATION OF E-CADHERIN DISTRIBUTION BY ENDOCYTOSIS [J].

de Beco, Simon ;

Amblard, Francois ;

Coscoy, Sylvie .

INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY, VOL 295, 2012, 295 :63-108

[7] Morphogenesis in the absence of integrins:: mutation of both Drosophila β subunits prevents midgut migration [J].

Devenport, D ;

Brown, NH .

DEVELOPMENT, 2004, 131 (21) :5405-5415

[8] Real-time imaging of morphogenetic movements in Drosophila using Gal4-UAS-driven expression of GFP fused to the actin-binding domain of moesin [J].

Dutta, D ;

Bloor, JW ;

Ruiz-Gomez, M ;

VijayRaghavan, K ;

Kiehart, DP .

GENESIS, 2002, 34 (1-2) :146-151

[9]

Grawe F, 1996, DEVELOPMENT, V122, P951

[10] Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in Drosophila [J].

Harris, TJC ;

Peifer, M .

JOURNAL OF CELL BIOLOGY, 2004, 167 (01) :135-147

← 1 2 3 4 →