Anthocyanins from Vitis coignetiae Pulliat Inhibit Cancer Invasion and Epithelial-Mesenchymal Transition, but These Effects Can Be Attenuated by Tumor Necrosis Factor in Human Uterine Cervical Cancer HeLa Cells

被引：18

作者：

Lu, Jing Nan ^{[1
,2
]}

Lee, Won Sup ^{[1
]}

Yun, JeongWon ^{[1
]}

Kim, Min Jeong ^{[1
]}

Kim, Hye Jung ^{[3
]}

Kim, Dong Chul ^{[4
]}

Jeong, Jae-Hoon ^{[5
]}

Choi, Yung Hyun ^{[6
,7
]}

Kim, Gon-Sup ^{[8
]}

Ryu, Chung Ho ^{[9
]}

Shin, Sung Chul ^{[10
]}

机构：

[1] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Dept Internal Med, Jinju 660702, South Korea

[2] Fifth Hosp Shijizhuang, Shijiazhuang 050021, Peoples R China

[3] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Dept Pharmacol, Jinju 660702, South Korea

[4] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Dept Pathol, Jinju 660702, South Korea

[5] Korea Inst Radiol & Med Sci, Div Radiat Canc Res, Seoul 660702, South Korea

[6] Dong Eui Univ, Coll Oriental Med, Dept Biochem, Pusan 614052, South Korea

[7] Dong Eui Univ, Grad Sch, Dept Biomat Control, Program BK21, Pusan 614052, South Korea

[8] Gyeongsang Natl Univ, Sch Vet Med, Jinju 660701, South Korea

[9] Gyeongsang Natl Univ, Inst Agr & Life Sci, Div Appl Life Sci, BK Program 21, Jinju 660701, South Korea

[10] Gyeongsang Natl Univ, Life Sci Res Inst, Dept Chem, Jinju 660701, South Korea

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 2013卷

关键词：

NF-KAPPA-B; MATRIX METALLOPROTEINASES; ALPHA-KINASE; E-CADHERIN; APOPTOSIS; PATHWAY; EXPRESSION; CYANIDIN-3-GLUCOSIDE; PROLIFERATION; SUPPRESSION;

D O I：

10.1155/2013/503043

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Recently we have demonstrated that anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) have anticancer effects. Here, we investigate the effects of AIMs on cell proliferation and invasion as well as epithelial-mesenchymal transition (EMT) which have been linked to cancer metastasis in human uterine cervical cancer HeLa cells. AIMs inhibited the invasion of HeLa cells in a dose-dependent manner. AIMs inhibited MMP-9 expression in a dose-dependent manner. AIMs inhibited the motility of HeLa cells in a wound healing test. AIMs still suppressed NF-kappa B activation induced by TNF. AIMs also inhibited EMT in HeLa cells. AIMs suppressed vimentin, N-cadherin, and beta-catenin expression and induced E-cadherin. AIMs also suppressed expression of beta-catenin and Snail, which was regulated by GSK-3. These effects of AIMs were also limited in the HeLa cells treated with TNF. In conclusion, this study indicates that AIMs have anticancer effects by suppressing NF-kappa B-regulated genes and EMT, which relates to suppression of I kappa B alpha. phosphorylation and GSK-3 activity, respectively. However, the effects of AIMs were attenuated in the TNF-high condition.

引用

页数：11

共 33 条

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