A high-fat diet induces obesity and impairs bone acquisition in young male mice

被引:44
作者
Lu, Xiao-Mei [1 ]
Zhao, Hong [2 ]
Wang, En-Hua [2 ]
机构
[1] China Med Univ, Coll Basic Med, Dept Pathophysiol, Shenyang 110001, Liaoning, Peoples R China
[2] China Med Univ, Inst Pathol & Pathophysiol, Shenyang 110001, Liaoning, Peoples R China
关键词
obesity; high-fat diet; Wnt; beta-catenin; osteoblast; MINERAL DENSITY; BODY-MASS; OSTEOPOROSIS; OVERWEIGHT; CHILDHOOD; WOMEN; RATS;
D O I
10.3892/mmr.2013.1297
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The postnatal development of obesity is highly associated with the excessive consumption of a high-calorie, high-fat diet (HFD). However, the correlation between HFD-induced pediatric obesity and skeletal development remains to be elucidated. In the present study, postnatal day 17 (PND17) mice were weaned on a HFD for eight weeks ad libitum to induce obesity. The HFD mice showed a significant increase in the total body weight and gonadal and abdominal fat mass compared with the control animals. Peripheral quantitative (pQ) CT scans of the tibial bone revealed that the bone mineral density (BMD), including the total, trabecular and cortical BMD, was unchanged between the HFD and control diet groups, but that it was inversely associated with body fat. By contrast, the bone mineral content (BMC) and trabecular area were significantly decreased in the HFD group compared with the control. RNA and protein were isolated from the femur. qPCR and western blot analyses showed a significant downregulation in the gene expression of the key canonical Wnt signaling molecule beta-catenin, the osteoblastic cell differentiation marker Runt-related transcription factor 2 (Runx2) and also in the beta-catenin gene encoded protein levels of the HFD mice when compared with the controls. Consistent with the increased fat mass in the HFD-induced obese animals, the expression of the adipogenic genes and aP2 was increased compared with the controls. Bone marrow cells were aspirated and the ex vivo bone marrow cell cultures showed that the number of colony-forming unit osteoblasts (CFU-OBs) per bone was significantly decreased in the samples from the HFD mice compared with those from the controls. These observations suggested that HFD-induced obesity in growing animals may affect the total available osteoblastic cell differentiation progenitors in the bone, while increasing adipogenesis. This may result in negative consequences for the bone later on in adult life.
引用
收藏
页码:1203 / 1208
页数:6
相关论文
共 25 条
  • [1] Childhood obesity and skeletal maturation assessed with Fishman's hand-wrist analysis
    Akridge, Matthew
    Hilgers, Kelly K.
    Silveira, Anibal M.
    Scarfe, William
    Scheetz, James P.
    Kinane, Denis F.
    [J]. AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS, 2007, 132 (02) : 185 - 190
  • [2] Short-Term Exposure to Low-Carbohydrate, High-Fat Diets Induces Low Bone Mineral Density and Reduces Bone Formation in Rats
    Bielohuby, Maximilian
    Matsuura, Maiko
    Herbach, Nadja
    Kienzle, Ellen
    Slawik, Marc
    Hoeflich, Andreas
    Bidlingmaier, Martin
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2010, 25 (02) : 275 - 284
  • [3] High-fat diet decreases cancellous bone mass but has no effect on cortical bone mass in the tibia in mice
    Cao, Jay J.
    Gregoire, Brian R.
    Gao, Hongwei
    [J]. BONE, 2009, 44 (06) : 1097 - 1104
  • [4] Musculoskeletal effects of obesity
    Chan, Gilbert
    Chen, Christopher T.
    [J]. CURRENT OPINION IN PEDIATRICS, 2009, 21 (01) : 65 - 70
  • [5] Obesity Reduces Bone Density Associated with Activation of PPARγ and Suppression of Wnt/β-Catenin in Rapidly Growing Male Rats
    Chen, Jin-Ran
    Lazarenko, Oxana P.
    Wu, Xianli
    Tong, Yudong
    Blackburn, Michael L.
    Shankar, Kartik
    Badger, Thomas M.
    Ronis, Martin J. J.
    [J]. PLOS ONE, 2010, 5 (10):
  • [6] Dietary-Induced Serum Phenolic Acids Promote Bone Growth via p38 MAPK/β-Catenin Canonical Wnt Signaling
    Chen, Jin-Ran
    Lazarenko, Oxana P.
    Wu, Xianli
    Kang, Jie
    Blackburn, Michael L.
    Shankar, Kartik
    Badger, Thomas M.
    Ronis, Martin J. J.
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2010, 25 (11) : 2399 - 2411
  • [7] Ethanol Impairs Estrogen Receptor Signaling Resulting in Accelerated Activation of Senescence Pathways, Whereas Estradiol Attenuates the Effects of Ethanol in Osteoblasts
    Chen, Jin-Ran
    Lazarenko, Oxana P.
    Haley, Rani Lynn
    Blackburn, Michael L.
    Badger, Thomas M.
    Ronis, Martin J. J.
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 2009, 24 (02) : 221 - 230
  • [8] Attenuation of the self-renewal of transit-amplifying osteoblast progenitors in the murine bone marrow by 17β-estradiol
    Di Gregorio, GB
    Yamamoto, M
    Ali, AA
    Abe, E
    Roberson, P
    Manolagas, SC
    Jilka, RL
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2001, 107 (07) : 803 - 812
  • [9] Obesity but not overweight is associated with increased mortality risk
    Faeh, David
    Braun, Julia
    Tarnutzer, Silvan
    Bopp, Matthias
    [J]. EUROPEAN JOURNAL OF EPIDEMIOLOGY, 2011, 26 (08) : 647 - 655
  • [10] EFFECTS OF WEIGHT AND BODY-MASS INDEX ON BONE-MINERAL DENSITY IN MEN AND WOMEN - THE FRAMINGHAM-STUDY
    FELSON, DT
    ZHANG, YQ
    HANNAN, MT
    ANDERSON, JJ
    [J]. JOURNAL OF BONE AND MINERAL RESEARCH, 1993, 8 (05) : 567 - 573