An AOTF-based dual-modality hyperspectral imaging system (DMHSI) capable of simultaneous fluorescence and reflectance imaging

被引：21
作者：
Martin, ME
Wabuyele, M
Panjehpour, M
Overholt, B
DeNovo, R
Kennel, S
Cunningham, G
Vo-Dinh, T
机构：
[1] Oak Ridge Natl Lab, Div Life Sci, Ctr Adv Biomed Photon, Oak Ridge, TN 37831 USA
[2] Tennessee Technol Univ, Dept Mech Engn, Cookeville, TN 38501 USA
[3] Thompson Canc Ctr, Knoxville, TN 37916 USA
[4] Univ Tennessee, Sch Vet, Dept Small Anim Clin Sci, Knoxville, TN 37996 USA
[5] Oak Ridge Natl Lab, Ctr Mammalian Genet, Oak Ridge, TN 37831 USA
来源：
MEDICAL ENGINEERING & PHYSICS | 2006年 / 28卷 / 02期
关键词：
hyperspectral fluorescence spectroscopy; acousto-optic tunable filters; medical imaging; biomedical optics; laser-induced fluorescence;
D O I：
10.1016/j.medengphy.2005.04.022
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
An acousto-optic tunable filter (AOTF)-based system for dual-modality hyperspectral imaging (DMHSI) has been developed for use in characterization of normal and malignant mouse tissue. The system consists of a laser, endoscope, AOTE and two cameras coupled with optics and electronics. Initial results show that the system can delineate normal and malignant mouse tissues real-time. The analysis shows that malignant tissues consistently exhibit less fluorescent intensity in the wavelength band from 440 to 540 nm with a peak intensity of around 490 nm. The analysis also shows key spectroscopic differences between normal and malignant tissues. Further, these results are compared to real-time spectroscopic data and show good correlation. (c) 2005 IPEM. Published by Elsevier Ltd. All rights reserved.
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收藏
页码：149 / 155
页数：7
相关论文
共 25 条
[1]   FLUORESCENCE IMAGING AND POINT MEASUREMENTS OF TISSUE - APPLICATIONS TO THE DEMARCATION OF MALIGNANT-TUMORS AND ATHEROSCLEROTIC LESIONS FROM NORMAL TISSUE [J].
ANDERSSONENGELS, S ;
JOHANSSON, J ;
SVANBERG, K ;
SVANBERG, S .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1991, 53 (06) :807-814
[2]   Use of acousto-optic tuneable filter in fluorescence imaging endoscopy [J].
Bouhifd, M ;
Whelan, M ;
Aprahamian, M .
NOVEL OPTICAL INSTRUMENTATION FOR BIOMEDICAL APPLICATIONS, 2003, 5143 :305-314
[3]   CCD CAMERA AS A MULTICHANNEL ANALYZER FOR THE SPECTRAL AND AZIMUTHAL RESOLUTION OF FABRY-PEROT FRINGES [J].
BUNTING, CA ;
CAROLAN, PG ;
FORREST, MJ ;
NOONAN, PG ;
SHARPE, AC .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1988, 59 (08) :1488-1490
[4]  
CHENG LJ, 1993, P SOC PHOTO-OPT INS, V1874, P224, DOI 10.1117/12.148065
[5]   FIBEROPTIC LASER-SCANNING CONFOCAL MICROSCOPE SUITABLE FOR FLUORESCENCE IMAGING [J].
DELANEY, PM ;
HARRIS, MR ;
KING, RG .
APPLIED OPTICS, 1994, 33 (04) :573-577
[6]   Biopsy surveillance is still necessary in patients with Barrett's oesophagus despite new endoscopic imaging techniques [J].
Egger, K ;
Werner, M ;
Meining, A ;
Ott, R ;
Allescher, HD ;
Höfler, H ;
Classen, M ;
Rösch, T .
GUT, 2003, 52 (01) :18-23
[7]  
EHRHARDT A, 2003, MED LASER APPL, V18, P27
[8]   ACOUSTOOPTIC SUPERMULTISPECTRAL IMAGING [J].
GAO, GH ;
LIN, Z .
APPLIED OPTICS, 1993, 32 (17) :3081-3086
[9]   In vivo autofluorescence imaging of early cancers in the human tracheobronchial tree with a spectrally optimized system [J].
Goujon, D ;
Zellweger, M ;
Radu, A ;
Grosjean, P ;
Weber, BC ;
van den Bergh, H ;
Monnier, P ;
Wagnières, G .
JOURNAL OF BIOMEDICAL OPTICS, 2003, 8 (01) :17-25
[10]  
GUPTA N, 1999, P SOC PHOTO-OPT INS, V3718, P1
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