Conservation and Multiplication of Encapsulated Micro Shoots of Rauvolfia vomitoria - an Endangered Medicinal Tree: ISSR and RAPD based Evaluation of Genetic Fidelity of Converted Plantlets

被引：0

作者：

Mehrotra, Shakti ^{[1
]}

Rahman, Liaq Ur ^{[1
]}

Mishra, Jahnvi ^{[1
]}

Kukreja, Arun K. ^{[1
]}

机构：

[1] CSIR, Dept Plant Biotechnol, Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India

来源：

NATURAL PRODUCT COMMUNICATIONS | 2012年 / 7卷 / 12期

关键词：

Encapsulation; Rauvolfia vomitoria; Germplasm conservation; Genetic fidelity; ISSR; RAPD analysis; MICROPROPAGATED PLANTS; REGENERATION; TIPS; L; PROPAGATION; SERPENTINA; STORAGE;

D O I：

暂无

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The in vitro grown axillary micro shoots of Rauvolfia vomitoria were encapsulated in alginate beads. Following 6 months of normal storage at 25 +/- 2 degrees C the regrowth of encapsulated micro shoots, reached 95.2% within 40 days of incubation on MS medium containing 1.0 mg/L BAP and 0.1 mg/L NAA. Among the responding encapsulated explants 69.6% showed emergence of multiple shoots. The developing shoots showed rhizogenesis in two weeks following their transfer to rooting medium. Healthy plants were established in a glass house with 95% survival. Of the 50 RAPD primers tested, 10 produced 23 clear and reproducible amplicons, with an average of 2.3 bands per primer. Eleven ISSR primers produced a total of 42 bands, with a size range of 0.1-1.9 kb. The number of scorable bands for each primer varied from 2 to 6, with an average of 3.81. The similarity matrix, calculated individually from the results obtained from ISSR and RAPD analysis, showed similarity coefficients ranging from 1.0 for RAPD and 0.85 to 1.0 for ISSR.

引用

页码：1647 / 1650

页数：4