The Origin of Stroma Surrounding Epithelial Ovarian Cancer Cells

被引:12
作者
Akahane, Tomoko
Hirasawa, Akira [1 ]
Tsuda, Hiroshi
Kataoka, Fumio
Nishimura, Sadako [2 ]
Tanaka, Hideo
Tominaga, Eiichiro
Nomura, Hiroyuki
Chiyoda, Tatsuyuki
Iguchi, Yoko
Yamagami, Wataru
Susumu, Nobuyuki
Aoki, Daisuke
机构
[1] Keio Univ, Dept Obstet & Gynecol, Sch Med, Shinjyuku Ku, Tokyo 1608582, Japan
[2] Osaka City Gen Hosp, Dept Obstet & Gynecol, Osaka, Japan
关键词
Ovarian cancer; Stroma; p53; BREAST-CANCER; TUMOR-STROMA; P53; PROTEIN; CARCINOMAS; MUTATIONS; IDENTIFICATION; FIBROBLASTS; BLOOD; TP53;
D O I
10.1097/PGP.0b013e3182518533
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Cancer stroma is thought to play an important role in tumor behavior, including invasion or metastasis and response to therapy. Cancer stroma is generally thought either to be non-neoplastic cells, including tissue-marrow or bone-marrow-derived fibroblasts, or to originate in epithelial mesenchymal transition of cancer cells. In this study, we evaluated the status of the p53 gene in both the cancer cells and the cancer stroma in epithelial ovarian cancer (EOC) to elucidate the origin of the stroma. Samples from 16 EOC patients were included in this study. Tumor cells and adjacent nontumor stromal cells were microdissected and DNA was extracted separately. We analyzed p53 sequences (exons 5-8) of both cancer and stromal tissues in all cases. Furthermore, we examined p53 protein expression in all cases. Mutations in p53 were detected in 9 of the 16 EOCs: in 8 of these cases, the mutations were detected only in cancer cells. In 1 case, the same mutation (R248Q) was detected in both cancer and stromal tissues, and p53 protein expression was detected in both the cancer cells and the cancer stroma. Most cancer stroma in EOC is thought to originate from non-neoplastic cells, but some parts of the cancer stroma might originate from cancer cells.
引用
收藏
页码:26 / 30
页数:5
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