Tequila vinasses acidogenesis in a UASB reactor with Clostridium predominance

被引:10
作者
Marino-Marmolejo, E. N. [1 ]
Corbala-Robles, L. [2 ]
Cortez-Aguilar, R. C. [2 ]
Contreras-Ramos, S. M. [2 ]
Bolanos-Rosales, R. E. [2 ]
Davila-Vazquez, G. [2 ]
机构
[1] Ctr Invest & Asistencia Tecnol & Diseno Estado Ja, Biotecnol Med & Farmaceut, Guadalajara 44270, Jalisco, Mexico
[2] Ctr Invest & Asistencia Tecnol & Diseno Estado Ja, Tecnol Ambiental, Guadalajara 44270, Jalisco, Mexico
来源
SPRINGERPLUS | 2015年 / 4卷
关键词
Stillage; Volatile fatty acid; Biohydrogen; DGGE; Agave; BIOHYDROGEN PRODUCTION; ANAEROBIC-DIGESTION; RETENTION TIME; HYDROGEN; FERMENTATION; TEMPERATURE; REMOVAL; PH;
D O I
10.1186/s40064-015-1193-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Tequila vinasses represent an acidic, highly concentrated pollutant effluent generated during the distillation step of Tequila production. Although acidogenesis of Tequila vinasses has been reported for some reactor configurations, a characterization of the bacteria present during this metabolic process is lacking in the literature. Hydraulic retention times (HRT) between 36 and 6 h and organic loading rates (OLR) from 5 to 30 g COD L-1 d(-1) were assessed in a UASB reactor fed with Tequila vinasses. Results showed that OLR excerted a stronger effect (p <= 0.0001) on parameters such as gas production rate, pH, and acidity than HRT. While it was clear that shorter HRT were related to higher volatile fatty acid production levels. Figures above 2 L-gas L-reactor(-1) d(-1) (where "gas" could be a mixture of methane and hydrogen) were attained only with an OLR as high as 30 g COD L-1 d(-1). Bacterial identification of a sludge sample at the end of the experiment revealed that acid-tolerant microorganisms that remained in the reactor were exclusively affiliated to the Clostridium genera, being the first report of organisms identification for Tequila vinasses acidogenesis. These findings are relevant to the field of biotechnology since acidogenesis of Tequila vinasses using identified and studied microorganism abilities (i.e. Clostridium strains) presents the opportunity of optimizing processes intended for different metabolites production (butanol, volatile fatty acids, hydrogen, solvents).
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页数:8
相关论文
共 30 条
  • [1] Butanol production from thin stillage using Clostridium pasteurianum
    Ahn, Jae-Hyung
    Sang, Byoung-In
    Urn, Youngsoon
    [J]. BIORESOURCE TECHNOLOGY, 2011, 102 (07) : 4934 - 4937
  • [2] [Anonymous], 2006, STANDARD METHODS EXA
  • [3] Acidogenic fermentation of industrial wastewaters: Effects of chemostat retention time and pH on volatile fatty acids production
    Bengtsson, Simon
    Hallquist, Jakob
    Werker, Alan
    Welander, Thomas
    [J]. BIOCHEMICAL ENGINEERING JOURNAL, 2008, 40 (03) : 492 - 499
  • [4] Biohydrogen production from tequila vinasses using a fixed bed reactor
    Buitron, German
    Prato-Garcia, Dorian
    Zhang, Axue
    [J]. WATER SCIENCE AND TECHNOLOGY, 2014, 70 (12) : 1919 - 1925
  • [5] Hydrogen and methane production via a two-stage processes (H2-SBR + CH4-UASB) using tequila vinasses
    Buitron, German
    Kumar, Gopalakrishnan
    Martinez-Arce, Andres
    Moreno, Gloria
    [J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2014, 39 (33) : 19249 - 19255
  • [6] Biohydrogen production from Tequila vinasses in an anaerobic sequencing batch reactor: Effect of initial substrate concentration, temperature and hydraulic retention time
    Buitron, German
    Carvajal, Carolina
    [J]. BIORESOURCE TECHNOLOGY, 2010, 101 (23) : 9071 - 9077
  • [7] Inhibition of anaerobic digestion process: A review
    Chen, Ye
    Cheng, Jay J.
    Creamer, Kurt S.
    [J]. BIORESOURCE TECHNOLOGY, 2008, 99 (10) : 4044 - 4064
  • [8] CNIT, 2015, INF BAS IND TEQ
  • [9] Consejo Regulador del Tequila (CRT), 2015, INF EST
  • [10] CRT, 2015, GEOGR TERR APP OR TD