Up-Regulation of circRNA hsa_circ_0003074 Expression is a Reliable Diagnostic and Prognostic Biomarker in Patients with Osteosarcoma

Objective: The purpose of this study was to detect the expression level of circRNA hsa_circ_0003074 in the tissues and peripheral blood of patients with osteosarcoma, and to explore its value in diagnosis and predicting prognosis of patients with osteosarcoma. Materials and Methods: Tissue samples of osteosarcoma patients for chip sequencing were collected and circRNA hsa_circ_0003074, which was associated with the most obvious differential expression, was selected for qRT-PCR analysis. At the same time, the expression levels of hsa_circ_0003074 in different osteosarcoma cell lines, and in the peripheral blood of osteosarcoma patients before and after chemotherapy or surgery, as well as those in healthy volunteers matched by age and sex were also tested. This study aimed to explore the relationship between hsa_circ_0003074 and clinical characteristics of patients with osteosarcoma and analyzed its impact on patient survival. Results: High expression of hsa_circ_0003074 was detected in osteosarcoma tissues, peripheral blood and osteosarcoma cell lines, and the expression level of hsa_circ_0003074 in the peripheral blood of patients with osteosarcoma decreased significantly after chemotherapy or surgery. In addition, hsa_circ_0003074 was closely related to clinical characteristics, such as tumor size, lung metastasis, enneking stage, and chemotherapy resistance. The ROC curve showed that hsa_circ_0003074 was a good diagnostic indicator for osteosarcoma, and survival analysis results revealed that high expression of hsa_circ_0003074 affected the survival of patients. Conclusion: hsa_circ_0003074 is not only highly expressed in the tissues and peripheral blood of osteosarcoma patients but also a reliable diagnostic and prognostic biomarker for osteosarcoma patients.