Enzymatic and Molecular Characterization of Arabidopsis ppGpp Pyrophosphohydrolase, AtNUDX26

被引：29

作者：

Ito, Daisuke ^{[1
]}

Kato, Takahiro ^{[1
]}

Maruta, Takanori ^{[1
,2
]}

Tamoi, Masahiro ^{[1
]}

Yoshimura, Kazuya ^{[3
]}

Shigeoka, Shigeru ^{[1
]}

机构：

[1] Kinki Univ, Fac Agr, Dept Adv Biosci, Nara 6318505, Japan

[2] Shimane Univ, Fac Life & Environm Sci, Matsue, Shimane 6908504, Japan

[3] Chubu Univ, Coll Biosci & Biotechnol, Dept Food & Nutr Sci, Kasugai, Aichi 4878501, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 12期

关键词：

Arabidopsis; ganosine; 3; 5; '-bispyrophosphate; (ppGpp); nucleoside diphosphates linked to some moiety X (Nudix) hydrolase; ADP-RIBOSE/NADH PYROPHOSPHOHYDROLASE; 5'-DIPHOSPHATE 3'-DIPHOSPHATE PPGPP; NUDIX HYDROLASES; ESCHERICHIA-COLI; GUANOSINE TETRAPHOSPHATE; BACTERIAL ALARMONE; GLOBAL REGULATOR; RNA-POLYMERASE; BETA-SUBUNIT; HIGHER-PLANT;

D O I：

10.1271/bbb.120523

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Not only in bacteria but also in plant cells, guanosine-3',5'-tetraphosphate (ppGpp) is an important signaling molecule, that affects various cellular processes. In this study, we identified nucleoside diphosphates linked to some moiety X (Nudix) hydrolases, AtNUDX11, 15, 25, and 26, having ppGpp pyrophosphohydrolase activity from Arabidopsis plants. Among these, AtNUDX26 localized in chloroplasts had the highest V-max and k(cat) values, leading to high catalytic efficiency, k(cat)/K-m. The activity of AtNUDX26 required Mg2+ or Mn2+ ions as cofactor and was optimal at pH 9.0 and 50 degrees C. The expression of AtNUDX26 and of ppGpp metabolism-associated genes was regulated by various types of stress, suggesting that AtNUDX26 regulates cellular ppGpp levels in response to stress and impacts gene expression in chloroplasts. This is the first report on the molecular properties of ppGpp pyrophosphohydrolases in plants.

引用

页码：2236 / 2241

页数：6

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