Dual-enzymatically crosslinked hyaluronic acid hydrogel as a long-time 3D stem cell culture system

被引：30

作者：

Gao, Feng ^{[1
]}

Li, Jinrui ^{[1
]}

Wang, Luyu ^{[1
]}

Zhang, Dan ^{[1
]}

Zhang, Junni ^{[1
]}

Guan, Fangxia ^{[1
,2
]}

Yao, Minghao ^{[1
]}

机构：

[1] Zhengzhou Univ, Sch Life Sci, 100 Sci Rd, Zhengzhou 450001, Peoples R China

[2] Henan Prov Peoples Hosp, Zhengzhou, Peoples R China

来源：

BIOMEDICAL MATERIALS | 2020年 / 15卷 / 04期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

hyaluronic acid hydrogel; dual-enzymatically crosslinking; long-time three-dimensional stem cell culture; FORMED IN-SITU; TYRAMINE HYDROGELS; SCAFFOLD;

D O I：

10.1088/1748-605X/ab712e

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Stem cell-based tissue engineering shows enormous potential for regenerative medicine. Three-dimensional (3D) stem cell culture is the most basic aspect of tissue engineering. However, achievement of a perfect scaffold for highly efficient 3D cell culture is currently still limited. Herein, a new hyaluronic acid hydrogel dual-enzymatically crosslinked by horseradish peroxidase and choline oxidase is developed as a 3D stem cell culture system. This hydrogel possesses superior stability over two months, controllable biodegradability with hyaluronidases, a high swelling ratio exceeding 6000%, and excellent cytocompatibilityin vitroand biocompatibilityin vivo. More importantly, a long-time and highly cellular activity 3D culture of bone marrow-derived mesenchymal stem cells was achievedin vitroover 20 days. All these encouraging results highlight the great potential of this new hydrogel for 3D culture and tissue engineering.

引用

页数：9