Dual-enzymatically crosslinked hyaluronic acid hydrogel as a long-time 3D stem cell culture system

被引：34
作者：
Gao, Feng [1 ]
Li, Jinrui [1 ]
Wang, Luyu [1 ]
Zhang, Dan [1 ]
Zhang, Junni [1 ]
Guan, Fangxia [1 ,2 ]
Yao, Minghao [1 ]
机构：
[1] Zhengzhou Univ, Sch Life Sci, 100 Sci Rd, Zhengzhou 450001, Peoples R China
[2] Henan Prov Peoples Hosp, Zhengzhou, Peoples R China
来源：
BIOMEDICAL MATERIALS | 2020年 / 15卷 / 04期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
hyaluronic acid hydrogel; dual-enzymatically crosslinking; long-time three-dimensional stem cell culture; FORMED IN-SITU; TYRAMINE HYDROGELS; SCAFFOLD;
D O I：
10.1088/1748-605X/ab712e
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Stem cell-based tissue engineering shows enormous potential for regenerative medicine. Three-dimensional (3D) stem cell culture is the most basic aspect of tissue engineering. However, achievement of a perfect scaffold for highly efficient 3D cell culture is currently still limited. Herein, a new hyaluronic acid hydrogel dual-enzymatically crosslinked by horseradish peroxidase and choline oxidase is developed as a 3D stem cell culture system. This hydrogel possesses superior stability over two months, controllable biodegradability with hyaluronidases, a high swelling ratio exceeding 6000%, and excellent cytocompatibilityin vitroand biocompatibilityin vivo. More importantly, a long-time and highly cellular activity 3D culture of bone marrow-derived mesenchymal stem cells was achievedin vitroover 20 days. All these encouraging results highlight the great potential of this new hydrogel for 3D culture and tissue engineering.
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页数：9
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