Isolation and characterization of microsatellites in Portunus trituberculatus

被引：8

作者：

Cui, Zhaoxia ^{[1
]}

Liu, Yuan ^{[1
]}

Wang, Hongxia ^{[1
]}

Wu, Danhua ^{[1
]}

Luan, Weisha ^{[1
]}

Tan, Feng ^{[1
]}

Huang, Minde ^{[1
]}

机构：

[1] Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2012年 / 4卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Portunus trituberculatus; Microsatellites; Polymorphism;

D O I：

10.1007/s12686-011-9518-0

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Thirty polymorphic microsatellite markers for swimming crab P. trituberculatus are developed from EST sequences, genomic libraries and cross-species amplification in P. pelagicus, respectively. They are characterized using 24 P. trituberculatus individuals from Yingkou natural population. The number of alleles per locus range from 4 to 21 with a mean number of 10.83. The observed and expected heterozygosities range from 0.250 to 1.000 and from 0.365 to 0.945, respectively. Five loci departed significantly from HWE (P < 0.05). The genomic-SSRs and EST-SSRs show similar levels of polymorphism for both the average number of alleles (11 alleles vs. 10 alleles) and average PIC values (PIC = 0.780 vs. PIC = 0.776). These microsatellite loci from different sources will contribute to improve research on the genetic conservation of P. trituberculatus.

引用

页码：251 / 255

页数：5

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