Molecular Evolution of Enterovirus 68 Detected in the Philippines

被引：32

作者：

Imamura, Tadatsugu ^{[1
]}

Suzuki, Akira ^{[1
]}

Lupisan, Socorro ^{[7
]}

Okamoto, Michiko ^{[1
]}

Aniceto, Rapunzel ^{[2
,3
]}

Egos, Rutchie J. ^{[3
]}

Daya, Edgardo E. ^{[4
]}

Tamaki, Raita ^{[2
]}

Saito, Mariko ^{[2
]}

Fuji, Naoko ^{[1
]}

Roy, Chandra Nath ^{[1
]}

Opinion, Jaime M. ^{[5
]}

Santo, Arlene V. ^{[6
]}

Macalalad, Noel G. ^{[7
]}

Tandoc, Amado, III ^{[7
]}

Sombrero, Lydia ^{[7
]}

Olveda, Remigio ^{[7
]}

Oshitani, Hitoshi ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Med, Sendai, Miyagi 980, Japan

[2] Tohoku RITM Res Ctr Emerging & Reemerging Infect, Muntinlupa, Philippines

[3] Eastern Visayas Reg Med Ctr, Tacloban City, Philippines

[4] Leyte Prov Hosp, Palo City, Philippines

[5] Tacloban City Hlth Catchment Ctr, Tacloban City, Philippines

[6] Tanuan Rural Hlth Unit, Tanuan City, Philippines

[7] Res Inst Trop Med, Muntinlupa, Philippines

来源：

PLOS ONE | 2013年 / 8卷 / 09期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

RESPIRATORY-INFECTIONS; HOSPITALIZED CHILDREN; RECOMBINATION; RHINOVIRUS; EMERGENCE; DYNAMICS;

D O I：

10.1371/journal.pone.0074221

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Detection of Enterovirus 68 (EV68) has recently been increased. However, underlying evolutionary mechanism of this increasing trend is not fully understood. Methods: Nasopharyngeal swabs were collected from 5,240 patients with acute respiratory infections in the Philippines from June 2009 to December 2011. EV68 was detected by polymerase chain reaction (PCR) targeting for 5' untranslated region (5' UTR), viral protein 1 (VP1), and VP4/VP2. Phylogenetic trees were generated using the obtained sequences. Results: Of the 5,240 tested samples, 12 EV68 positive cases were detected between August and December in 2011 (detection rate, 0.23%). The detection rate was higher among inpatients than outpatients (p<0.0001). Among VP1 sequences detected from 7 patients in 2011, 5 in lineage 2 were diverged from those detected in the Philippines in 2008, however, 2 in lineage 3 were not diverged from strains detected in the Philippines in 2008 but closely associated with strains detected in the United States. Combined with our previous report, EV68 occurrences were observed twice in the Philippines within the last four years. Conclusions: EV68 detections might be occurring in cyclic patterns, and viruses might have been maintained in the community while some strains might have been newly introduced.

引用

页数：7

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