Rapid analysis of transgenic rice straw using near-infrared spectroscopy

被引:39
作者
Hattori, Takefumi [1 ]
Murakami, Shinya [1 ]
Mukai, Mai [1 ]
Yamada, Tatsuhiko [2 ]
Hirochika, Hirohiko [3 ]
Ike, Masakazu [4 ]
Tokuyasu, Ken [4 ]
Suzuki, Shiro [1 ]
Sakamoto, Masahiro [5 ]
Umezawa, Toshiaki [1 ,6 ]
机构
[1] Kyoto Univ, Res Inst Sustainable Humanosphere, Uji, Kyoto 6110011, Japan
[2] Forestry & Forest Prod Res Inst, Tsukuba, Ibaraki 3058687, Japan
[3] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan
[4] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan
[5] Kyoto Univ, Grad Sch Agr, Kyoto 6068502, Japan
[6] Kyoto Univ, Inst Sustainabil Sci, Uji, Kyoto 6110011, Japan
关键词
Lignin; near-infrared spectroscopy; starch; enzymatic saccharification efficiency; CHEMICAL-COMPOSITION; LIGNIN CONTENT; WOOD; BIOSYNTHESIS; PREDICTION; FEEDSTOCK; CELLULOSE; FRUCTOSE; GLUCOSE; TREES;
D O I
10.5511/plantbiotechnology.12.0501a
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A rapid near-infrared (NIR) spectroscopy method was established to predict the lignin and starch contents and enzymatic saccharification efficiency of transgenic rice (Oryza sativa cv. Nipponbare) straw wherein expression of genes encoding lignin synthetic enzymes are regulated. Strong correlations were obtained between laboratory wet chemistry values and the NIR-predicted values. This method is useful to develop transgenic rice where cell-wall formation is engineered.
引用
收藏
页码:359 / 366
页数:8
相关论文
共 42 条
[1]  
Agarwal UP, 2010, LIGNIN LIGNANS ADV C, P103, DOI [10.1201/EBK1574444865-c4, 10.1016/0924-2031(90)80020-5]
[2]   Structural and chemical properties of grass lignocelluloses related to conversion for biofuels [J].
Anderson, William F. ;
Akin, Danny E. .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2008, 35 (05) :355-366
[3]   Translational genomics for bioenergy production: There's room for more than one model [J].
Bush, Daniel R. ;
Leach, Jan E. .
PLANT CELL, 2007, 19 (10) :2971-2973
[4]   Lignin modification improves fermentable sugar yields for biofuel production [J].
Chen, Fang ;
Dixon, Richard A. .
NATURE BIOTECHNOLOGY, 2007, 25 (07) :759-761
[5]   Monolignol biosynthesis and genetic engineering of lignin in trees, a review [J].
Chiang, Vincent L. .
ENVIRONMENTAL CHEMISTRY LETTERS, 2006, 4 (03) :143-146
[6]   Transgenics are imperative for biofuel crops [J].
Gressel, Jonathan .
PLANT SCIENCE, 2008, 174 (03) :246-263
[7]   EFFICIENT TRANSFORMATION OF RICE (ORYZA-SATIVA L) MEDIATED BY AGROBACTERIUM AND SEQUENCE-ANALYSIS OF THE BOUNDARIES OF THE T-DNA [J].
HIEI, Y ;
OHTA, S ;
KOMARI, T ;
KUMASHIRO, T .
PLANT JOURNAL, 1994, 6 (02) :271-282
[8]   Contribution of the Tos17 retrotransposon to rice functional genomics [J].
Hirochika, H .
CURRENT OPINION IN PLANT BIOLOGY, 2001, 4 (02) :118-122
[9]   Insertional mutagenesis with Tos17 for functional analysis of rice genes [J].
Hirochika, Hirohiko .
BREEDING SCIENCE, 2010, 60 (05) :486-492
[10]   Genetic modification of lignin biosynthesis for improved biofuel production [J].
Hisano, Hiroshi ;
Nandakumar, Rangaraj ;
Wang, Zeng-Yu .
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2009, 45 (03) :306-313