We describe an ultrasensitive immunoassay for detecting biotoxins that uses liposomes with encapsulated DNA reporters, and ganglioside receptors embedded in the bilayer, as a detection reagent. After immobilization of the target biotoxin by a capture antibody and co-binding of the detection reagent, the liposomes are ruptured to release the reporters, which are quantified by real-time PCR. Assays for cholera and botulinum toxins are several orders of magnitude more sensitive than current detection methods.
机构:
Queen Mary Univ London, Acad Dept Surg, St Bartholomews & Royal London Sch Med & Dent, London E1 1BB, EnglandQueen Mary Univ London, Acad Dept Surg, St Bartholomews & Royal London Sch Med & Dent, London E1 1BB, England
机构:
Queen Mary Univ London, Acad Dept Surg, St Bartholomews & Royal London Sch Med & Dent, London E1 1BB, EnglandQueen Mary Univ London, Acad Dept Surg, St Bartholomews & Royal London Sch Med & Dent, London E1 1BB, England