A liposome-PCR assay for the ultrasensitive detection of biological toxins

被引:104
作者
Mason, JT
Xu, LX
Sheng, ZM
O'Leary, TJ
机构
[1] Armed Forces Inst Pathol, Dept Biophys, Rockville, MD 20850 USA
[2] Armed Forces Inst Pathol, Dept Mol Pathol, Rockville, MD 20850 USA
[3] Vet Hlth Adm, Biomed Lab Res & Dev Serv, Washington, DC 20420 USA
关键词
D O I
10.1038/nbt1201
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We describe an ultrasensitive immunoassay for detecting biotoxins that uses liposomes with encapsulated DNA reporters, and ganglioside receptors embedded in the bilayer, as a detection reagent. After immobilization of the target biotoxin by a capture antibody and co-binding of the detection reagent, the liposomes are ruptured to release the reporters, which are quantified by real-time PCR. Assays for cholera and botulinum toxins are several orders of magnitude more sensitive than current detection methods.
引用
收藏
页码:555 / 557
页数:3
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