Formation of artificial lipid bilayers using droplet dielectrophoresis

被引:70
作者
Aghdaei, Sara [1 ]
Sandison, Mairi E. [1 ]
Zagnoni, Michele [1 ]
Green, Nicolas G. [1 ]
Morgan, Hywel [1 ]
机构
[1] Univ Southampton, Sch Elect & Comp Sci, Southampton, Hants, England
关键词
D O I
10.1039/b807374k
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid-alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.
引用
收藏
页码:1617 / 1620
页数:4
相关论文
共 20 条
[1]   Detection of single ion channel activity on a chip using tethered bilayer membranes [J].
Andersson, Martin ;
Keizer, Henk M. ;
Zhu, Chenyu ;
Fine, Daniel ;
Dodabalapur, Ananth ;
Duran, Randolph S. .
LANGMUIR, 2007, 23 (06) :2924-2927
[2]   Controlled microfluidic interfaces [J].
Atencia, J ;
Beebe, DJ .
NATURE, 2005, 437 (7059) :648-655
[3]   Heterodimer formation and crystal nucleation of gramicidin D [J].
Burkhart, BM ;
Gassman, RM ;
Langs, DA ;
Pangborn, WA ;
Duax, WL .
BIOPHYSICAL JOURNAL, 1998, 75 (05) :2135-2146
[4]   Single ion channel sensitivity in suspended bilayers on micromachined supports [J].
Cheng, YL ;
Bushby, RJ ;
Evans, SD ;
Knowles, PF ;
Miles, RE ;
Ogier, SD .
LANGMUIR, 2001, 17 (04) :1240-1242
[5]   Concentration and binary separation of micro particles for droplet-based digital microfluidics [J].
Cho, Sung Kwon ;
Zhao, Yuejun ;
Kim, Chang-Jin CJ'' .
LAB ON A CHIP, 2007, 7 (04) :490-498
[6]   Digital microfluidics: is a true lab-on-a-chip possible? [J].
Fair, R. B. .
MICROFLUIDICS AND NANOFLUIDICS, 2007, 3 (03) :245-281
[7]   Dielectrophoresis-based programmable fluidic processors [J].
Gascoyne, PRC ;
Vykoukal, JV ;
Schwartz, JA ;
Anderson, TJ ;
Vykoukal, DM ;
Current, KW ;
McConaghy, C ;
Becker, FF ;
Andrews, C .
LAB ON A CHIP, 2004, 4 (04) :299-309
[8]   Direct detection of membrane channels from gels using water-in-oil droplet bilayers [J].
Heron, Andrew J. ;
Thompson, James R. ;
Mason, Amy E. ;
Wallace, Mark I. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (51) :16042-16047
[9]   Functional bionetworks from nanoliter water droplets [J].
Holden, Matthew A. ;
Needham, David ;
Bayley, Hagan .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (27) :8650-8655
[10]   Asymmetric droplet interface bilayers [J].
Hwang, William L. ;
Chen, Min ;
Cronin, Brid ;
Holden, Matthew A. ;
Bayley, Hagan .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (18) :5878-+