Over-expression of recombinant proteins with N-terminal His-tag via subcellular uneven distribution in Escherichia coli

被引:19
|
作者
Park, Won-Ji [1 ]
You, Sung-Hwan [1 ]
Choi, Hyoung-An [1 ]
Chu, Yeon-Jin [1 ]
Kim, Geun-Joong [1 ]
机构
[1] Chonnam Natl Univ, Coll Nat Sci, Dept Biol Sci, Kwangju 500757, South Korea
关键词
His-tag; over-expression; uneven distribution; immunofluorescence; BACTERIAL-CELL; OVEREXPRESSION; LOCALIZATION; PURIFICATION; TOXIN; RNA;
D O I
10.1093/abbs/gmv036
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Specific tags with defined amino acid residues are widely used to purify or probe target proteins. Interestingly, the tagging system occasionally results in an increase of the recombinant protein expression in vivo. Here, we systematically examined this phenomenon using a poly-histidine (His)-tag fused to N- or C-terminal region of green, red, and blue fluorescent proteins by quantification and uneven distribution in cytoplasm of Escherichia coli. This effect was further supported by the distinct over-expression of several unrelated proteins, such as esterase, neopullulanase, and chloramphenicol acetyltransferase, tagging with the same tag. These results suggest that a poly-His-tag placed at N-terminal region can induce over-expression of recombinant protein via subcellular uneven distribution in vivo.
引用
收藏
页码:488 / 495
页数:8
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