Instantaneous fluorescent probe for the specific detection of H2S

被引：43

作者：

Lin, Xianfeng ^{[1
]}

Lu, Xiuhong ^{[1
]}

Zhou, Junliang ^{[1
]}

Ren, Hang ^{[1
]}

Dong, Xiaochun ^{[1
]}

Zhao, Weili ^{[1
,2
]}

Chen, Zhongjian ^{[3
]}

机构：

[1] Fudan Univ, Sch Pharm, 826 Zhangheng Rd, Shanghai 201203, Peoples R China

[2] Henan Univ, Minist Educ, Key Lab Special Funct Mat, Kaifeng 475004, Peoples R China

[3] Shanghai Dermatol Hosp, Shanghai 200443, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2019年 / 213卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; H2S; Lysosome-targeted; ENDOGENOUS HYDROGEN-SULFIDE; LIVING CELLS; SELECTIVE DETECTION; RAPID DETECTION; BLOOD-PLASMA; MITOCHONDRIA; LYSOSOME;

D O I：

10.1016/j.saa.2019.01.085

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Novel cyanine-based fluorescent probes for the detection of H2S were developed. The probes developed are stable under physiological conditions. The water soluble fluorescent probe 2 displayed ultrafast and specific response to H2S displaying NIR fluorescence of 115-fold turn-on with the detection limit of 11 nM without assistance of organic solvent or surfactant. Cell imaging experiments indicated that probe 2 was cellpermeable and was able to detect H2S sensitively in lysosomes. Moreover, our probe was able to detect H2S intrinsically produced H2S through enzymatic/non-enzymatic biosynthetic pathway from Cys/GSH. Moreover, we applied probe 2 to detect H2S in living mice and demonstrated the fast metabolism of H2S. Thus, probe 2 shows great promise as a reporter for H2S. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：416 / 422

页数：7

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