Molecular separation of Oesophagostomum stephanostomum and Oesophagostomum bifurcum (Nematoda: Strongyloidea) from non-human primates

被引：27
作者：
Gasser, RB
Woods, WG
Huffman, MA
Blotkamp, J
Polderman, AM
机构：
[1] Univ Melbourne, Dept Vet Sci, Werribee, Vic 3030, Australia
[2] Kyorin Univ, Primate Res Inst, Sect Ecol, Inuyama, Aichi 4848506, Japan
[3] Leiden Univ, Fac Med, Dept Parasitol, NL-2300 RC Leiden, Netherlands
来源：
INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1999年 / 29卷 / 07期
基金：
英国惠康基金; 澳大利亚研究理事会;
关键词：
nematoda; Oesophagostomum stephanostomum; Oesophagostomum bifurcum; non-human primates; second internal transcribed spacer (ITS-2);
D O I：
10.1016/S0020-7519(99)00037-5
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The ITS-2 sequences for adult specimens of Oesophagostomum stephanostomum from the common chimpanzee and Oesophagostomum bifurcum? from the Mona monkey were determined. For both species, the length and GC content of the ITS-2 sequences were 216 bp and 43%, respectively. While there was no unequivocal sequence difference among individual worms representing each of the two species, five (2.3%) interspecific nucleotide differences were detected. These differences were associated with the presence of unique restriction sites in the ITS-2 sequence of O. stephanostomum for multiple endonucleases of diagnostic value for the differentiation of the two taxa by restriction analysis. Pairwise comparisons of the ITS-2 sequences of O. stephanostomum and O. bifurcum with published ITS-2 sequences for five different congeners indicated that these species from the subgenus Conoweberia are closely related, in accordance with previous morphological studies. (C) 1999 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
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收藏
页码：1087 / 1091
页数：5
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