Detection of Natural Toxoplasma gondii Infection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction

被引：21

作者：

Mose, John Mokua ^{[1
,2
]}

Kagira, John Maina ^{[3
]}

Karanja, Simon Muturi ^{[2
]}

Ngotho, Maina ^{[4
]}

Kamau, David Muchina ^{[2
]}

Njuguna, Adele Nyambura ^{[2
]}

Maina, Naomi Wangari ^{[5
]}

机构：

[1] Kenya Methodist Univ, Sch Med & Hlth Sci, Dept Med Lab Sci, POB 45240, Nairobi 00100, Kenya

[2] JKUAT, Dept Publ Hlth, POB 62000, Nairobi 00200, Kenya

[3] Jomo Kenyatta Univ Agr & Technol JKUAT, Dept Anim Sci, POB 62000, Nairobi 00200, Kenya

[4] Mt Kenya Univ, Dept Anim Hlth & Prod, POB 342, Thika 01000, Kenya

[5] JKUAT, Dept Biochem, POB 62000, Nairobi 00200, Kenya

来源：

BIOMED RESEARCH INTERNATIONAL | 2016年 / 2016卷

关键词：

PREVALENCE; SEROPREVALENCE; PITFALLS; HORMONES; DISEASE; PUNJAB; RISK;

D O I：

10.1155/2016/7589278

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Thedetection of Toxoplasma gondii in free-range chickens is a good indicator of possible risk to human beings. The aim of this study was to investigate the occurrence of T. gondii in free-range chicken using polymerase chain reaction (PCR). Brain samples from 105 free-range chickens fromthree administrative areas inThika region, Kenya, were collected, DNA-extracted, and analyzed using PCR to detect presence of T. gondii. The overall prevalence of T. gondii in all the three areas was 79.0% (95% CI: 70.0-86.4%) and the prevalence across the three areas was not significantly different (P = 0.5088; chi(2) = 1.354). Female chickens had higher (79.4%) prevalence than males (78.6%), although the difference was not significant (P = 0.922, chi(2) = 0.01). However, chickens that were more than 2 years old had significantly (P = 0.003; chi(2) = 11.87) higher prevalence compared to younger ones. The study indicates that there was a high occurrence of T. gondii infection in free-range chickens from Thika region and that the infection rate is age dependent. Further studies should be carried out to determine the possible role of roaming chickens in the epidemiology of the disease among humans in the area.

引用

页数：5

共 30 条

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