Single-cell adhesion of human osteoblasts on plasma-conditioned titanium implant surfaces in vitro

被引：9

作者：

Becker, Merlind ^{[1
]}

Schmied, Frederike ^{[1
]}

Kadem, Laith F. ^{[2
]}

Freitag-Wolf, Sandra ^{[3
,4
]}

Naujokat, Hendrik ^{[4
]}

Mehl, Christian ^{[1
]}

Kern, Matthias ^{[1
]}

Harder, Sonke ^{[1
]}

机构：

[1] Christian Albrechts Univ Kiel, Dept Prosthodont Propaedeut & Dent Mat, Arnold Heller Str 16, D-24105 Kiel, Germany

[2] Christian Albrechts Univ Kiel, Inst Mat Sci & Biocompatible Nanomat, Kaiserstr 2, D-24143 Kiel, Germany

[3] Christian Albrechts Univ Kiel, Inst Med Informat & Stat, Brunswiker Str 10, D-24105 Kiel, Germany

[4] Christian Albrechts Univ Kiel, Dept Oral & Maxillofacial Surg, Arnold Heller Str 16, D-24105 Kiel, Germany

来源：

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS | 2020年 / 109卷

关键词：

Physical plasma; Osteoblasts; Titanium; Dental implants; Initial single-cell adhesion; Single-cell force spectroscopy; ATOMIC-FORCE MICROSCOPY; DENTAL IMPLANTS; EARLY OSSEOINTEGRATION; ATMOSPHERIC PLASMA; ULTRAVIOLET-LIGHT; ARGON; WETTABILITY; ENERGY; HYDROPHILICITY; TOPOGRAPHY;

D O I：

10.1016/j.jmbbm.2020.103841

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Objectives: This study aimed to demonstrate the effect of treating titanium-implant surfaces with plasma from two different sources on wettability and initial single-cell adhesion of human osteoblasts and to investigate whether aging affects treatment outcomes. Methods: Titanium disks with sandblasted and acid-etched (SLA) surfaces were treated with atmospheric pressure plasma (APP) and low-pressure plasma (LPP). For wetting behavior of the specimens after plasma treatment, the water contact angle was measured. The single-cell detachment force and amount of work of detachment of human osteoblasts were determined with single-cell force spectroscopy (SCFS). To evaluate the aging effect in APP-treated specimens, SCFS was conducted 10 and 60 min after treatment. Results: Significantly higher hydrophilicity was observed in the APP and LPP treatment groups than in the control group, but no significant difference was observed between the APP and LPP groups. No significant difference in cell-detachment force or work of detachment was observed, and there were no significant differences according to the conditioning mechanisms and storage time. Significance: Conditioning of the titanium surfaces with APP or LPP was not a significant influencing factor in the initial adhesion of the osteoblasts.

引用

页数：8