N-Myc downstream-regulated gene 2 is involved in p53-mediated apoptosis

被引：73

作者：

Liu, Na ^{[1
]}

Wang, Lifeng ^{[1
]}

Li, Xia ^{[1
]}

Yang, Qi ^{[1
]}

Liu, Xinping ^{[1
]}

Zhang, Jing ^{[1
]}

Zhang, Jian ^{[1
]}

Wu, Yousheng ^{[1
]}

Ji, Shaoping ^{[1
]}

Zhang, Yingqi ^{[2
]}

Yang, Angang ^{[3
]}

Han, Hua ^{[4
]}

Yao, Libo ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Dept Biochem & Mol Biol, State Key Lab Canc Biol, Xian 710032, Peoples R China

[2] Fourth Mil Med Univ, Ctr Biotechnol, Xian 710032, Peoples R China

[3] Fourth Mil Med Univ, Dept Immunol, Xian 710032, Peoples R China

[4] Fourth Mil Med Univ, Dept Med Genet & Dev Biol, Xian 710032, Peoples R China

来源：

NUCLEIC ACIDS RESEARCH | 2008年 / 36卷 / 16期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

D O I：

10.1093/nar/gkn504

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tumor suppressor, p53, is a transcription factor which can modulate the transcription of a number of target genes that are involved in cell-cycle arrest and apoptosis. However, the apoptotic pathway mediated by p53 is not fully understood. Here, we showed that N-Myc downstream-regulated gene 2 (NDRG2) is a new target gene that is regulated by p53. NDRG2 mRNA and protein levels can be upregulated in a p53-dependent manner. The first intron of the NDRG2 gene contains a site that binds p53 directly and mediates wild-type p53-dependent transactivation. In addition, silencing of NDRG2 attenuates p53-mediated apoptosis, whereas overexpression of NDRG2 suppresses tumor cell growth, regardless of the presence or absence of p53. Our results indicate that NDRG2 is a novel p53-inducible target that is involved in the p53-mediated apoptosis pathway.

引用

页码：5335 / 5349

页数：15

共 60 条

[1] Identification of SCN3B as a novel p53-inducible proapoptotic gene [J].

Adachi, K ;

Toyota, M ;

Sasaki, Y ;

Yamashita, T ;

Ishida, S ;

Ohe-Toyota, M ;

Maruyama, R ;

Hinoda, Y ;

Saito, T ;

Imai, K ;

Kudo, R ;

Tokino, T .

ONCOGENE, 2004, 23 (47) :7791-7798

[2] Cellular differentiation determines the expression of the hypoxia-inducible protein NDRG1 in pancreatic cancer [J].

Angst, E. ;

Sibold, S. ;

Tiffon, C. ;

Weimann, R. ;

Gloor, B. ;

Candinas, D. ;

Stroka, D. .

BRITISH JOURNAL OF CANCER, 2006, 95 (03) :307-313

[3] Role of the putative tumor metastasis suppressor gene Drg-1 in breast cancer progression [J].

Bandyopadhyay, S ;

Pai, SK ;

Hirota, S ;

Hosobe, S ;

Takano, Y ;

Saito, K ;

Piquemal, D ;

Commes, T ;

Watabe, M ;

Gross, SC ;

Wang, Y ;

Ran, S ;

Watabe, K .

ONCOGENE, 2004, 23 (33) :5675-5681

[4]

Bandyopadhyay S, 2003, CANCER RES, V63, P1731

[5] p53-dependent pathways of apoptosis [J].

Benchimol, S .

CELL DEATH AND DIFFERENTIATION, 2001, 8 (11) :1049-1051

[6] MDM2 is a central node in the p53 pathway: 12 years and counting [J].

Bond, GL ;

Hu, WW ;

Levine, AJ .

CURRENT CANCER DRUG TARGETS, 2005, 5 (01) :3-8

[7] Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane [J].

Bourdon, JC ;

Renzing, J ;

Robertson, PL ;

Fernandes, KN ;

Lane, DP .

JOURNAL OF CELL BIOLOGY, 2002, 158 (02) :235-246

[8] N-Myc down-regulated gene 1 modulates the response of term human trophoblasts to hypoxic injury [J].

Chen, B ;

Nelson, DM ;

Sadovsky, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (05) :2764-2772

[9] Dissecting p53-dependent apoptosis [J].

Chipuk, J. E. ;

Green, D. R. .

CELL DEATH AND DIFFERENTIATION, 2006, 13 (06) :994-1002

[10] Expression and regulation of NDRG2 (N-myc downstream regulated gene 2) during the differentiation of dendritic cells [J].

Choi, SC ;

Kim, KD ;

Kim, JT ;

Kim, JW ;

Yoon, DY ;

Choe, YK ;

Chang, YS ;

Paik, SG ;

Lim, JS .

FEBS LETTERS, 2003, 553 (03) :413-418

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