Generation and Characterization of Monoclonal Antibodies Against Rift Valley Fever Virus Nucleoprotein

被引：4
|
作者：
Fafetine, J. M. [1 ,2 ]
Domingos, A. [3 ]
Antunes, S. [4 ]
Esteves, A. [4 ]
Paweska, J. T. [5 ]
Coetzer, J. A. W. [6 ,7 ]
Rutten, V. P. M. G. [6 ,7 ]
Neves, L. [1 ,2 ]
机构：
[1] Eduardo Mondlane Univ, Fac Vet, Maputo, Mozambique
[2] Eduardo Mondlane Univ, Ctr Biotechnol, Maputo, Mozambique
[3] Univ Nova Lisboa, Ctr Malaria & Other Trop Dis, Inst Hyg & Trop Med, P-1349008 Lisbon, Portugal
[4] Univ Nova Lisboa, Unidade Parasitol & Microbiol Med, Inst Higiene & Med Trop, P-1349008 Lisbon, Portugal
[5] Natl Hlth Lab Serv, Natl Inst Communicable Dis, Ctr Emerging & Zoonot Dis, Johannesburg, South Africa
[6] Univ Pretoria, Fac Vet Sci, Dept Vet Trop Dis, ZA-0110 Onderstepoort, South Africa
[7] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Utrecht, Netherlands
来源：
TRANSBOUNDARY AND EMERGING DISEASES | 2013年 / 60卷
关键词：
Rift Valley fever virus; nucleocapsid protein; monoclonal antibodies; cross-reactivity; LINKED-IMMUNOSORBENT-ASSAY; NUCLEOCAPSID PROTEIN; INDIRECT ELISA; RECOMBINANT NUCLEOPROTEIN; DOMESTIC RUMINANTS; AFRICAN BUFFALO; RAPID DIAGNOSIS; IGM ANTIBODIES; HUMAN-SERA; N-PROTEIN;
D O I：
10.1111/tbed.12140
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Due to the unpredictable and explosive nature of Rift Valley fever (RVF) outbreaks, rapid and accurate diagnostic assays for low-resource settings are urgently needed. To improve existing diagnostic assays, monoclonal antibodies (MAbs) specific for the nucleocapsid protein of RVF virus (RVFV) were produced and characterized. Four IgG2a MAbs showed specific binding to denatured nucleocapsid protein, both from a recombinant source and from inactivated RVFV, in Western blot analysis and in an enzyme-linked immunosorbent assay (ELISA). Cross-reactivity with genetically related and non-related arboviruses including Bunyamwera and Calovo viruses (Bunyaviridae family), West Nile and Dengue-2 viruses (Flaviviridae family), and Sindbis and Chikungunya viruses (Togaviridae family) was not detected. These MAbs represent a useful tool for the development of rapid diagnostic assays for early recognition of RVF.
引用
收藏
页码：24 / 30
页数：7
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