mRNA Localization Mechanisms in Trypanosoma cruzi

被引：11

作者：

Alves, Lysangela R. ^{[1
]}

Guerra-Slompo, Eloise P. ^{[1
]}

de Oliveira, Arthur V. ^{[1
]}

Malgarin, Juliane S. ^{[1
]}

Goldenberg, Samuel ^{[1
]}

Dallagiovanna, Bruno ^{[1
]}

机构：

[1] Fiocruz Parana, Inst Carlos Chagas, Lab Regulacao Expressao Genica, Curitiba, Parana, Brazil

来源：

PLOS ONE | 2013年 / 8卷 / 12期

关键词：

BINDING PROTEIN; DIFFERENTIATION; PROTEOMICS;

D O I：

10.1371/journal.pone.0081375

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Asymmetric mRNA localization is a sophisticated tool for regulating and optimizing protein synthesis and maintaining cell polarity. Molecular mechanisms involved in the regulated localization of transcripts are widespread in higher eukaryotes and fungi, but not in protozoa. Trypanosomes are ancient eukaryotes that branched off early in eukaryote evolution. We hypothesized that these organisms would have basic mechanisms of mRNA localization. FISH assays with probes against transcripts coding for proteins with restricted distributions showed a discrete localization of the mRNAs in the cytoplasm. Moreover, cruzipain mRNA was found inside reservosomes suggesting new unexpected functions for this vacuolar organelle. Individual mRNAs were also mobilized to RNA granules in response to nutritional stress. The cytoplasmic distribution of these transcripts changed with cell differentiation, suggesting that localization mechanisms might be involved in the regulation of stage-specific protein expression. Transfection assays with reporter genes showed that, as in higher eukaryotes, 3'UTRs were responsible for guiding mRNAs to their final location. Our results strongly suggest that Trypanosoma cruzi have a core, basic mechanism of mRNA localization. This kind of controlled mRNA transport is ancient, dating back to early eukaryote evolution.

引用

页数：10

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