5-Formylcytosine can be a stable DNA modification in mammals

被引：180

作者：

Bachman, Martin ^{[1
,2
]}

Uribe-Lewis, Santiago ^{[2
]}

Yang, Xiaoping ^{[2
]}

Burgess, Heather E. ^{[3
]}

Iurlaro, Mario ^{[3
]}

Reik, Wolf ^{[3
,4
]}

Murrell, Adele ^{[2
]}

Balasubramanian, Shankar ^{[1
,2
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

[2] Univ Cambridge, Canc Res UK Cambridge Inst, Cambridge, England

[3] Babraham Inst, Babraham, England

[4] Wellcome Trust Sanger Inst, Hinxton, England

来源：

NATURE CHEMICAL BIOLOGY | 2015年 / 11卷 / 08期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

SINGLE-BASE RESOLUTION; 5-HYDROXYMETHYLCYTOSINE; DEMETHYLATION; TET; 5-CARBOXYLCYTOSINE; 5-METHYLCYTOSINE; GENOME; METHYLATION; DEAMINATION; PROTEINS;

D O I：

10.1038/nchembio.1848

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in mouse DNA differ from those of 5-hydroxymethylcytosine (5hmC), and using stable isotope labeling in vivo, we show that 5fC can be a stable DNA modification. These results suggest that 5fC has functional roles in DNA that go beyond being a demethylation intermediate.

引用

页码：555 / U40

页数：4

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