Validated, reversed phase high performance liquid chromatography method for the estimation of etoposide in bulk and formulations

Reversed phase high performance liquid chromatographic method was developed and validated for the estimation of etoposide in bulk and formulations using UV detector. Selected mobile phase was a combination of 50 mM ammonium acetate buffer (pH 6.0) and methanol ( 50: 50 % v/v) and wavelength selected was 240 nm. Retention time of etoposide was 6.7 min. Linearity of the method was found to be 50 to 1000 ng/ml, with the regression coefficient of 0.9998. This method was validated according to ICH guidelines. Quantification was done by calculating area of the peak and the detection limit and quantitation limits were 10.34 ng/ml and 33.62 ng/ml respectively. There was no significant difference in the intra day and inter day analysis of etoposide determined for three different concentrations using this method. Present method can be applied for the determination of etoposide in quality control samples, formulations without interference of the excipients present and in the dissolution studies of the drug.