In vivo study on the monoamine neurotransmitters and their metabolites change in the striatum of Parkinsonian rats by liquid chromatography with an acetylene black nanoparticles modified electrode

被引:12
作者
Lin, Li [1 ,2 ]
Yang, Jie [2 ]
Lin, Ruipo [2 ]
Yu, Li [2 ]
Gao, Hongchang [2 ]
Yang, Shulin [1 ]
Li, Xiaokun [1 ,2 ]
机构
[1] Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China
[2] Wenzhou Med Coll, Dept Pharm, Wenzhou 325035, Peoples R China
关键词
Monoamine neurotransmitters; HPLC-electrochemical detection; Acetylene black nanoparticles; Chemically modified electrode; In vivo microdialysis; Parkinson's Disease; GLASSY-CARBON ELECTRODE; ELECTROCHEMICAL DETECTION; VOLTAMMETRIC DETERMINATION; DOPAMINE; BRAIN; MICRODIALYSIS; DISEASE; HPLC; MODELS;
D O I
10.1016/j.jpba.2012.09.011
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The variation in the concentration of monoamine neurotransmitters and their metabolites in an experimental Parkinsonian animal model established by unilateral 6-hydroxydopamine administration was studied. For the purpose of detecting monoamine neurotransmitters and their metabolites more sensitively, an acetylene black nanoparticles modified electrode was fabricated and used as the working electrode for an electrochemical detector in HPLC. The results indicated that the modified electrode exhibited efficiently electrocatalytic oxidation for monoamine neurotransmitters and their metabolites with relatively high sensitivity, long life, and stability. The linear ranges spanned four orders of magnitude (r > 0.998) and the detectability was on the level of 0.1 nmol L-1. The percent relative standard deviation (%RSD) for each compound at all concentration levels was lower than 2.57% and 1.94% for intra-day and inter-day precision, respectively. The mean recovery values were between 98.75% and 105.25%, and the %RSD was found to be less than 1.02%. Coupled with in vivo microdialysis sampling, the validated method was successfully applied to measure monoamine neurotransmitters and their metabolites in both sides of the striatum of conscious and freely moving Parkinsonian rats, and the extracellular monoamine neurotransmitters and their metabolites in the lesioned-side striatum of unilateral 6-hydroxydopamine-lesioned rats were lower than that in the intact side striatum or in the striatum of control rats. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:74 / 79
页数:6
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