Raman spectroscopy of proteins: a review

被引：899

作者：

Rygula, A. ^{[1
]}

Majzner, K. ^{[1
]}

Marzec, K. M. ^{[2
]}

Kaczor, A. ^{[1
,2
]}

Pilarczyk, M. ^{[1
]}

Baranska, M. ^{[1
,2
]}

机构：

[1] Jagiellonian Univ, Fac Chem, PL-30060 Krakow, Poland

[2] Jagiellonian Univ, Jagiellonian Ctr Expt Therapeut, PL-30348 Krakow, Poland

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2013年 / 44卷 / 08期

关键词：

secondary structure; alpha-helix; beta-sheet; FT-Raman; resonance Raman; 3-DIMENSIONAL FOURIER SYNTHESIS; HUMAN TRIOSEPHOSPHATE ISOMERASE; PANCREATIC TRYPSIN-INHIBITOR; NORMAL-COORDINATE ANALYSIS; ENHANCED RESONANCE RAMAN; BOVINE SERUM-ALBUMIN; CARBONIC-ANHYDRASE-B; AMINO-ACID-SEQUENCE; X-RAY-DIFFRACTION; OPTICAL-ACTIVITY;

D O I：

10.1002/jrs.4335

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

In this work, 26 proteins of different structure, function and properties are investigated by Raman spectroscopy with 488, 532 and 1064nm laser lines. The excitation lines were chosen in NIR and Vis range as the most common and to show the difference due to normal and resonance effect, sometimes accompanied by the fluorescence. The selected proteins were divided, according to the Structural Classification of Proteins, into four classes according to their secondary structure, i.e. -helical (), -sheet (), mixed structures (/, +, s) and others. For all compounds, FT-Raman and two Vis spectra are presented along with the detailed band assignment. To the best of our knowledge, this is the first review showing the potential of Raman spectroscopy for the measurement and analysis of such a large collection of individual proteins. This work can serve as a comprehensive vibrational spectra library, based on our and previous Raman measurements. Copyright (c) 2013 John Wiley & Sons, Ltd.

引用

页码：1061 / 1076

页数：16

共 139 条

[1] RESONANCE RAMAN-SPECTRA OF OCTAETHYLPORPHYRINATO-NI(II) AND MESO-DEUTERATED AND N-15 SUBSTITUTED DERIVATIVES .2. NORMAL COORDINATE ANALYSIS [J].