The J- and G/F-domains of the majorSynechocystisDnaJ protein Sll0897 are sufficient for cell viability but not for heat resistance

Hsp70 proteins and their Hsp40 co-chaperones are essential components of cellular chaperone networks in both prokaryotes and eukaryotes. Here, we performed a genetic analysis to define the protein domains required for the key functions of the major Hsp40/DnaJ protein Sll0897 of the cyanobacteriumSynechocystissp. PCC6803. The expression of the N-terminally located J- and G/F-domains is essential and sufficient for the proteins' fundamentalin vivofunctions, whereas the presence of the full-length protein, containing the C-terminal substrate-binding domains, is crucial under stress conditions.