Advances in protease engineering for laundry detergents

被引：84

作者：

Vojcic, Ljubica ^{[1
]}

Pitzler, Christian ^{[1
]}

Koerfer, Georgette ^{[1
]}

Jakob, Felix ^{[2
]}

Martinez, Ronny ^{[1
,3
]}

Maurer, Karl-Heinz ^{[4
]}

Schwaneberg, Ulrich ^{[1
,2
]}

机构：

[1] Rhein Westfal TH Aachen, D-52074 Aachen, Germany

[2] DWI Leibniz Inst Interact Mat, D-52074 Aachen, Germany

[3] EW Nutr GmbH, Enzyme Technol, D-50829 Cologne, Germany

[4] AB Enzymes GmbH, D-64293 Darmstadt, Germany

来源：

NEW BIOTECHNOLOGY | 2015年 / 32卷 / 06期

关键词：

SUBTILISIN CARLSBERG; DIRECTED EVOLUTION; ALKALINE PROTEASE; CRYSTAL-STRUCTURE; COLD ADAPTATION; AQUALYSIN I; ENZYME; SEQUENCE; CATALYSIS; PERHYDROLYSIS;

D O I：

10.1016/j.nbt.2014.12.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteases are essential ingredients in modern laundry detergents. Over the past 30 years, subtilisin proteases employed in the laundry detergent industry have been engineered by directed evolution and rational design to tailor their properties towards industrial demands. This comprehensive review discusses recent success stories in subtilisin protease engineering. Advances in protease engineering for laundry detergents comprise simultaneous improvement of thermal resistance and activity at low temperatures, a rational strategy to modulate pH profiles, and a general hypothesis for how to increase promiscuous activity towards the production of peroxycarboxylic acids as mild bleaching agents. The three protease engineering campaigns presented provide in-depth analysis of protease properties and have identified principles that can be applied to improve or generate enzyme variants for industrial applications beyond laundry detergents.

引用

页码：629 / 634

页数：6

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