Long non-coding RNA regulates hair follicle stem cell proliferation and differentiation through PI3K/AKT signal pathway

Long non-coding RNAs (lncRNAs) are defined as non-coding transcripts (>200 nucleotides) that serve important roles in the proliferation and differentiation of stem cells. Hair follicle stem cells (HFTs) have multidirectional differentiation potential and are able to differentiate into skin, hair follicles and sebaceous glands, serving a role in skin wound healing. The aim of the present study was to analyze the regulatory role of lncRNA AK015322 (IncRNA5322) in HFTs and the potential mechanism of IncRNA5322-mediated differentiation of HFTs. The results demonstrated that lncRNA5322 transfection promoted proliferation and differentiation in HFTs. It was identified that lncRNA5322 transfection upregulated the expression and phosphorylation of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) in HFTs. It was also observed that lncRNA5322 transfection upregulated microRNA (miR)-21 and miR-21 agonist (agomir-21) eliminated lncRNA5322-induced expression and phosphorylation of PI3K and AKT. The present study also demonstrated that agomir-21 blocked IncRNA5322-induced expression and phosphorylation of PI3K and AKT in HFTs. The results indicated that agomir-21 transfection also suppressed the IncRNA5322-induced proliferation and differentiation of HFTs. In conclusion, the results of the present study suggest that lncRNA5322 is able to promote the proliferation and differentiation of HFTs by targeting the miR-21-mediated PI3K-AKT signaling pathway in HFTs.