Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes

被引:21
作者
Bi, Kuo [1 ]
Yang, Jing [1 ]
Wang, Lei [1 ,2 ]
Gu, Yuan [1 ]
Zhan, Bin [3 ]
Zhu, Xinping [1 ]
机构
[1] Capital Med Univ, Dept Parasitol, Sch Basic Med Sci, Beijing, Peoples R China
[2] Capital Med Univ, Beijing Friendship Hosp, Beijing, Peoples R China
[3] Baylor Coll Med, Dept Pediat, Natl Sch Trop Med, Houston, TX 77030 USA
基金
中国国家自然科学基金;
关键词
SERINE-PROTEASE; HUMAN TRICHINOSIS; RAPID EXPULSION; MICE; IDENTIFICATION; INFECTIONS; EXPRESSION; ANTIGENS; VACCINES; CLONING;
D O I
10.1371/journal.pone.0136189
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background Trichinella spiralis infection induces protective immunity against re-infection in animal models. Identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Trichinella infection and immunodiagnosis. Methods and Findings The T. spiralis adult cDNA library was immunoscreened with sera from pigs experimentally infected with 20,000 infective T. spiralis larvae. Total 43 positive clones encoding for 28 proteins were identified; one of the immunodominant proteins was 20 kDa Ts-ES-1 secreted by Trichinella stichocytes and existing in the excretory/secretory (ES) products of T. spiralis adult and muscle larval worms. Ts-ES-1 contains 172 amino acids with a typical signal peptide in the first 20 amino acids. The expression of Ts-ES-1 was detected in both the adult and muscle larval stages at the mRNA and protein expression levels. Mice immunized with recombinant Ts-ES-1 (rTs-ES-1) formulated with ISA50v2 adjuvant exhibited a significant worm reduction in both the adult worm (27%) and muscle larvae burden (42.1%) after a challenge with T. spiralis compared to the adjuvant control group (p<0.01). The rTs-ES-1-induced protection was associated with a high level of specific anti-Ts-ES-1 IgG antibodies and a Th1/Th2 mixed immune response. Conclusion The newly identified rTs-ES-1 is an immunodominant protein secreted by Trichinella stichocytes during natural infection and enables to the induction of partial protective immunity in vaccinated mice against Trichinella infection. Therefore, rTs-ES-1 is a potential candidate for vaccine development against trichinellosis.
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页数:16
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