Evaluation of a single-step multiplex RT-PCR for influenza virus type and subtype detection in respiratory samples

被引：17

作者：

Poddar, SK ^{[1
]}

Espina, R

Schnurr, DP

机构：

[1] Univ Calif San Diego, Dept Pediat, Pediat Pharmacol Res Unit, La Jolla, CA 92093 USA

[2] Calif Dept Hlth Serv, Div Communicable Dis Control, Viral & Rickettsial Dis Lab, Richmond, CA USA

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2002年 / 16卷 / 03期

关键词：

influenza virus; multiplex PCR; typing; subtyping;

D O I：

10.1002/jcla.10036

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A single-step multiplex reverse transcription-polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza virus type and subtypes was evaluated for its use in clinical specimens. One part of each specimen was tested using an established standard culture and/or monoclonal antibody-based immunofluorescence assay method, and the other part was tested by the multiplex RT-PCR method for the presence or absence of the influenza virus, and its type and subtype. Sixty-seven specimens were examined. The results revealed a strong agreement between the data obtained by the established method and those obtained by the multiplex RTPCR. (C) 2002 Wiley-Liss, Inc.

引用

页码：163 / 166

页数：4

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