Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells

被引：18

作者：

Poettler, Marina ^{[1
]}

Staicu, Andreas ^{[1
]}

Zaloga, Jan ^{[1
]}

Unterweger, Harald ^{[1
]}

Weigel, Bianca ^{[1
]}

Schreiber, Eveline ^{[1
]}

Hofmann, Simone ^{[2
]}

Wiest, Irmi ^{[2
]}

Jeschke, Udo ^{[2
]}

Alexiou, Christoph ^{[1
]}

Janko, Christina ^{[1
]}

机构：

[1] Univ Hosp Erlangen, Sect Expt Oncol & Nanomed, Dept Otorhinolaryngol Head & Neck Surg, Else Kroner Fresenius Stiftung Professorship, D-91054 Erlangen, Germany

[2] Univ Munich, Dept Obstet & Gynecol, D-80337 Munich, Germany

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2015年 / 16卷 / 11期

关键词：

superparamagnetic iron oxide nanoparticles; protein corona; cancer therapy and diagnosis; reproductive health; granulosa cells; MAGNETIC NANOPARTICLES; OXIDATIVE STRESS; FLOW-CYTOMETRY; BIOCOMPATIBILITY; BIODISTRIBUTION; MICRONUCLEI; ACTIVATION; PARTICLES; RADICALS; AGENTS;

D O I：

10.3390/ijms161125960

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEONLA-BSA), or with dextran (SEONDEX). Both micronuclei testing and the detection of H2A.X revealed no genotoxic effects of SEONLA-BSA, SEONDEX or SEONLA. Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.

引用

页码：26280 / 26290

页数：11

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