Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells

被引:108
作者
West, J
Tompkins, CK
Balantac, N
Nudelman, E
Meengs, B
White, T
Bursten, S
Coleman, J
Kumar, A
Singer, JW
Leung, DW
机构
[1] CELL THERAPEUT INC, SEATTLE, WA 98119 USA
[2] LOUISIANA STATE UNIV, MED CTR, DEPT BIOCHEM & MOL BIOL, NEW ORLEANS, LA 70112 USA
关键词
D O I
10.1089/dna.1997.16.691
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lysophosphatidic acid (LPA) and phosphatidic acid (PA) are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. LPA acyltransferase (LPAAT), also known as 1-acyl sn-glycerol-3-phosphate acetyltransferase (EC 2.3.1.51), catalyzes the conversion of LPA to PA, In this study, we describe the isolation and characterization of two human cDNAs that encode proteins possessing LPAAT activities, These two proteins, designated here as LPAAT-alpha and LPAAT-beta, contain extensive sequence sequence similarities to microbial or plant LPAAT sequences, LPAAT-alpha mRNA was detected in all tissues with highest expression in skeletal muscle whereas LPAAT-beta was expressed predominantly in heart and liver tissues, Expression of these two cDNAs in an Escherichia coli strain with a mutated LPAAT gene (plsC) complements its growth defect and shifts the equilibrium of cellular lipid content from LPA to PA and other lipids. Overexpression of these two cDNAs in mammalian cells leads to increased LPAAT activity in cell-free extracts using an in vitro assay that measures the conversion of fluorescently labeled LPA to PA, This increase in LPAAT activity correlates with enhancement of transcription and synthesis of tumor necrosis factor-alpha and interleukin-6 from cells upon stimulation with interleukin-1 beta, suggesting LPAAT overexpression may amplify cellular signaling responses from cytokines.
引用
收藏
页码:691 / 701
页数:11
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