A bHLH-Type Transcription Factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1, Acts as a Repressor to Negatively Regulate Jasmonate Signaling in Arabidopsis

被引:270
作者
Nakata, Masaru [1 ]
Mitsuda, Nobutaka [1 ]
Herde, Marco [2 ]
Koo, Abraham J. K. [2 ]
Moreno, Javier E. [2 ]
Suzuki, Kaoru [1 ]
Howe, Gregg A. [2 ]
Ohme-Takagi, Masaru [1 ,3 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki 3058562, Japan
[2] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA
[3] Saitama Univ, Inst Environm Sci & Technol, Saitama 3388770, Japan
基金
美国国家卫生研究院;
关键词
GENE-EXPRESSION; ACID BIOSYNTHESIS; JAZ REPRESSORS; ABSCISIC-ACID; ANTHOCYANIN ACCUMULATION; STAMEN DEVELOPMENT; ANTHER DEHISCENCE; PLANT DEFENSE; MALE-STERILE; L-ISOLEUCINE;
D O I
10.1105/tpc.113.111112
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loop-helix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.
引用
收藏
页码:1641 / 1656
页数:16
相关论文
共 79 条
[1]   Role of Arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression [J].
Abe, H ;
YamaguchiShinozaki, K ;
Urao, T ;
Iwasaki, T ;
Hosokawa, D ;
Shinozaki, K .
PLANT CELL, 1997, 9 (10) :1859-1868
[2]   Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis [J].
Anderson, JP ;
Badruzsaufari, E ;
Schenk, PM ;
Manners, JM ;
Desmond, OJ ;
Ehlert, C ;
Maclean, DJ ;
Ebert, PR ;
Kazan, K .
PLANT CELL, 2004, 16 (12) :3460-3479
[3]   Jasmonate signalling network in Arabidopsis thaliana:: crucial regulatory nodes and new physiological scenarios [J].
Balbi, Virginia ;
Devoto, Alessandra .
NEW PHYTOLOGIST, 2008, 177 (02) :301-318
[4]   Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis [J].
Boter, M ;
Ruíz-Rivero, O ;
Abdeen, A ;
Prat, S .
GENES & DEVELOPMENT, 2004, 18 (13) :1577-1591
[5]   EXPRESSION VECTORS FOR AFFINITY PURIFICATION AND RADIOLABELING OF PROTEINS USING ESCHERICHIA-COLI AS HOST [J].
CHEN, BPC ;
HAI, TW .
GENE, 1994, 139 (01) :73-75
[6]   Gibberellin Acts through Jasmonate to Control the Expression of MYB21, MYB24, and MYB57 to Promote Stamen Filament Growth in Arabidopsis [J].
Cheng, Hui ;
Song, Susheng ;
Xiao, Langtao ;
Soo, Hui Meng ;
Cheng, Zhiwei ;
Xie, Daoxin ;
Peng, Jinrong .
PLOS GENETICS, 2009, 5 (03)
[7]   The bHLH Transcription Factor MYC3 Interacts with the Jasmonate ZIM-Domain Proteins to Mediate Jasmonate Response in Arabidopsis [J].
Cheng, Zhiwei ;
Sun, Li ;
Qi, Tiancong ;
Zhang, Bosen ;
Peng, Wen ;
Liu, Yule ;
Xie, Daoxin .
MOLECULAR PLANT, 2011, 4 (02) :279-288
[8]   The JAZ family of repressors is the missing link in jasmonate signalling [J].
Chini, Andrea ;
Fonseca, S. ;
Fernandez, G. ;
Adie, B. ;
Chico, J. M. ;
Lorenzo, O. ;
Garcia-Casado, G. ;
Lopez-Vidriero, I. ;
Lozano, F. M. ;
Ponce, M. R. ;
Micol, J. L. ;
Solano, R. .
NATURE, 2007, 448 (7154) :666-+
[9]   Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in response to wounding and herbivory [J].
Chung, Hoo Sun ;
Koo, Abraham J. K. ;
Gao, Xiaoli ;
Jayanty, Sastry ;
Thines, Bryan ;
Jones, A. Daniel ;
Howe, Gregg A. .
PLANT PHYSIOLOGY, 2008, 146 (03) :952-964
[10]   Alternative splicing expands the repertoire of dominant JAZ repressors of jasmonate signaling [J].
Chung, Hoo Sun ;
Cooke, Thomas F. ;
DePew, Cody L. ;
Patel, Lalita C. ;
Ogawa, Narihito ;
Kobayashi, Yuichi ;
Howe, Gregg A. .
PLANT JOURNAL, 2010, 63 (04) :613-622