Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp KI72

被引：6
|
作者：
Nagai, Keisuke [1 ]
Yasuhira, Kengo [1 ]
Tanaka, Yusuke [1 ]
Kato, Dai-ichiro [1 ]
Takeo, Masahiro [1 ]
Higuchi, Yoshiki [2 ,3 ]
Negoro, Seiji [1 ]
Shibata, Naoki [2 ,3 ]
机构：
[1] Univ Hyogo, Grad Sch Engn, Dept Mat Sci & Chem, Himeji, Hyogo 6712280, Japan
[2] Univ Hyogo, Grad Sch Life Sci, Dept Life Sci, Kamigori, Hyogo 6781297, Japan
[3] RIKEN SPring 8 Ctr, Sayo, Hyogo 6795248, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2013年 / 69卷
基金：
日本学术振兴会;
关键词：
OLIGOMER DEGRADATION GENE; 6-AMINOHEXANOATE-DIMER HYDROLASE; CRYSTALLOGRAPHIC ANALYSIS; PLASMID POAD2; CARBOXYLESTERASE; ENZYMES;
D O I：
10.1107/S1744309113024263
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Nylon hydrolase (NylC) encoded by Arthrobacter plasmid pOAD2 (NylC(p2)) was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylC(p2) was crystallized by the sitting-drop vapour-diffusion method with ammonium sulfate as a precipitant in 0.1 M HEPES buffer pH 7.5 containing 0.2 M NaCl and 25% glycerol. Diffraction data were collected from the native crystal to a resolution of 1.60 angstrom. The obtained crystal was spindle shaped and belonged to the C-centred orthorhombic space group C222(1), with unit-cell parameters a = 70.84, b = 144.90, c = 129.05 angstrom. A rotation and translation search gave one clear solution containing two molecules per asymmetric unit.
引用
收藏
页码：1151 / 1154
页数：4
相关论文
共 40 条
  • [1] Crystallization and X-ray diffraction analysis of nylon-oligomer hydrolase (NylC) from Agromyces sp KY5R
    Yasuhira, Kengo
    Shibata, Naoki
    Tanaka, Yasuhito
    Kumagai, Naoya
    Tanaka, Yusuke
    Nagai, Keisuke
    Kato, Dai-ichiro
    Takeo, Masahiro
    Negoro, Seiji
    Higuchi, Yoshiki
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2011, 67 : 892 - 895
  • [2] X-ray crystallographic and mutational analysis of the NylC precursor: catalytic mechanism of autocleavage and substrate hydrolysis of nylon hydrolase
    Negoro, Seiji
    Shibata, Naoki
    Kato, Dai-ichiro
    Tanaka, Yusuke
    Yasuhira, Kengo
    Nagai, Keisuke
    Oshima, Shohei
    Furuno, Yoko
    Yokoyama, Risa
    Miyazaki, Kaito
    Takeo, Masahiro
    Hengphasatporn, Kowit
    Shigeta, Yasuteru
    Lee, Young-Ho
    Higuchi, Yoshiki
    FEBS JOURNAL, 2023, 290 (13) : 3400 - 3421
  • [3] Crystallization and preliminary X-ray diffraction analysis of cold-active β-galactosidase from Arthrobacter sp C2-2
    Petroková, H
    Vondrácková, E
    Skálová, T
    Dohnálek, J
    Lipovová, P
    Spiwok, V
    Strnad, H
    Králová, B
    Hasek, J
    COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 2005, 70 (01) : 124 - 132
  • [4] Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis
    Ju, Hansol
    Pandian, Ramesh
    Kim, Kyungmin
    Kim, Kyeong Kyu
    Kim, T. Doohun
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2014, 70 : 473 - 475
  • [5] Crystallization and preliminary X-ray analysis of L-azetidine-2-carboxylate hydrolase from Pseudomonas sp strain A2C
    Toyoda, Mayuko
    Jitsumori, Keiji
    Mikami, Bunzo
    Wackett, Lawrence P.
    Kurihara, Tatsuo
    Esaki, Nobuyoshi
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 801 - 804
  • [6] Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis
    Ueda, Miruku
    Shimosaka, Makoto
    Arai, Ryoichi
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 : 1516 - 1520
  • [7] Crystallization and X-ray diffraction studies of cellobiose phosphorylase from Cellulomonas uda
    Van Hoorebeke, Annelies
    Stout, Jan
    Kyndt, John
    De Groeve, Manu
    Dix, Ina
    Desmet, Tom
    Soetaert, Wim
    Van Beeumen, Jozef
    Savvides, Savvas N.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 346 - 351
  • [8] Crystallization, X-ray diffraction analysis and phasing of carboxylesterase PA3859 from Pseudomonas aeruginosa
    Pesaresi, A
    Lamba, D
    BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2005, 1752 (02): : 197 - 201
  • [9] Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum
    Higashi, Yasuhiro
    Smith, Thomas J.
    Jez, Joseph M.
    Kutchan, Toni M.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 : 163 - 166
  • [10] SpaB, an atypically adhesive basal pilin from the lactobacillar SpaCBA pilus: crystallization and X-ray diffraction analysis
    Megta, Abhin Kumar
    Palva, Airi
    von Ossowski, Ingemar
    Krishnan, Vengadesan
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2019, 75 : 731 - 737
1234