MicroRNA-98 targets HMGA2 to inhibit the development of retinoblastoma through mediating Wnt/β-catenin pathway

被引：23

作者：

Li, Wei ^{[1
]}

Wang, Junmei ^{[2
]}

Zhang, Dongqing ^{[3
]}

Zhang, Xiting ^{[4
]}

Xu, Jumei ^{[5
]}

Zhao, Li ^{[6
]}

机构：

[1] Jining 1 Peoples Hosp, Dept Ophthalmol, Jining, Shandong, Peoples R China

[2] Rizhao Hosp TCM, Dept Clin Lab, Rizhao, Shandong, Peoples R China

[3] Peoples Hosp, Dept Publ Hlth, Zhangqiu Area, Jinan, Shandong, Peoples R China

[4] Peoples Hosp, Dept Ward, Zhangqiu Area, Jinan, Shandong, Peoples R China

[5] Peoples Hosp, Dept Hepatobiliary Gastrointestinal, Zhangqiu Area, Jinan, Shandong, Peoples R China

[6] Yankuang New Journey Gen Hosp, Dept Ophthalmol, 560 Kuangjian Dong Rd, Zoucheng 273500, Shandong, Peoples R China

来源：

CANCER BIOMARKERS | 2019年 / 25卷 / 01期

关键词：

miR-98; retinoblastoma; HMGA2; wnt/beta-catenin pathway; MOBILITY GROUP A2; CELL-PROLIFERATION; INVASION; MIGRATION; SUPPRESSES; CANCER; MIR-98; GROWTH; EXPRESSION; OVEREXPRESSION;

D O I：

10.3233/CBM-182315

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

BACKGROUND: Recently, the incidence and mortality of retinoblastoma (RB) have gradually increased. Many studies support the pivotal role of microRNAs (miRNAs) in the pathogenesis of RB. Alternation of microRNA-98 (miR-98) expression has been detected in several cancers, excluding RB. This study was designed to assess the regulatory mechanisms of miR-98 in human RB. METHODS: RT-qPCR and Western blot analysis were used to detect miR-98 and HMGA2 expression. The effects of miR-98 were explored using the CCK-8 and Transwell assays. Dual-luciferase reporter assay was performed to confirm the relationship between miR-98 and HMGA2. RESULTS: In RB, downregulation of miR-98 was identified. Upregulation of miR-98 inhibited proliferation, invasion and migration of RB cells. Further, HMGA2 was confirmed as a direct target gene of miR-98. And knockdown of HMGA2 suppressed the progression of RB. Moreover, upregulation of HMGA2 reversed the suppressive effects in the development of RB. In addition, miR-98 also showed suppressive effect on EMT and Wnt/beta-catenin pathway. CONCLUSION: MiR-98 targets HMGA2 to act as a tumor suppressor in RB by mediating Wnt/beta-catenin pathway.

引用

页码：79 / 88

页数：10