Three-dimensional imaging of micro-specimen by optical scanning holography

被引：1
|
作者：
Liu, Jung-Ping [1 ]
Tsou, Cheng-Hao [1 ]
机构：
[1] Feng Chia Univ, Dept Photon, 100 Wenhwa Rd, Taichung 40724, Taiwan
来源：
BIOMEDICAL IMAGING AND SENSING CONFERENCE | 2017年 / 10251卷
关键词：
optical scanning holography; OSH; digital holography; DH; microscopy; DIGITAL HOLOGRAPHY;
D O I：
10.1117/12.2269503
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Optical scanning holography (OSH) is a scanning-type digital holographic technique. In OSH, a heterodyne interference pattern is generated to raster scan the object. OSH can be operated in the incoherent mode and thus is able to record a fluorescence hologram. In addition, resolution of the OSH is proportional to the density of the interference pattern. Here we use a high-NA microscope objective to generate a dynamic Fresnel zone plate to record a hologram of micro-specimen. The achieved transverse resolution and longitudinal resolution are 0.78 mu m and 3.1 mu m, respectively.
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页数：2
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