Human platelets attenuate Aspergillus species via granule-dependent mechanisms

被引：59

作者：

Perkhofer, Susanne ^{[1
]}

Kehrel, Beate E. ^{[4
,5
]}

Dierich, Manfred P. ^{[1
]}

Donnelly, J. Peter ^{[7
]}

Nussbaumer, Walter ^{[2
]}

Hofmann, Johann ^{[3
]}

von Eiff, Christof ^{[6
]}

Lass-Florl, Cornelia ^{[1
]}

机构：

[1] Innsbruck Med Univ, Dept Hyg Microbiol & Social Med, A-6020 Innsbruck, Austria

[2] Innsbruck Med Univ, Dept Transfus Med, A-6020 Innsbruck, Austria

[3] Innsbruck Med Univ, Div Med Biochem, A-6020 Innsbruck, Austria

[4] Univ Hosp, Dept Anesthesiol & Intens Care, Munster, Germany

[5] Univ Hosp, Dept Expt & Clin Hemostasis, Munster, Germany

[6] Univ Hosp, Dept Med Microbiol, Munster, Germany

[7] Radboud Univ Nijmegen, Med Ctr, Dept Hematol, NL-6525 ED Nijmegen, Netherlands

来源：

JOURNAL OF INFECTIOUS DISEASES | 2008年 / 198卷 / 08期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1086/591458

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Using laser scanning microscopy, we investigated whether platelets are capable of internalizing Aspergillus conidia and examined Aspergillus-platelet adherence. The influence of platelets on fungal growth was evaluated by assessing galactomannan (GM) release, hyphal elongation, and colony size. A secretion assay with [H-3]-serotonin (5-hydroxytryptamine [5-HT]) was performed. Exposure to platelets resulted in significantly decreased GM release (P <.05), hyphal elongation (P <.001), colony size, pigmentation, and 5-HT release (P <.05). A lack of antifungal effects was observed with the microfilament inhibitor cytochalasin D. Platelets attenuate the virulence of Aspergillus species in vitro on the basis of granule-dependent effects.

引用

页码：1243 / 1246

页数：4

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