Human platelets attenuate Aspergillus species via granule-dependent mechanisms

被引：61

作者：

Perkhofer, Susanne ^{[1
]}

Kehrel, Beate E. ^{[4
,5
]}

Dierich, Manfred P. ^{[1
]}

Donnelly, J. Peter ^{[7
]}

Nussbaumer, Walter ^{[2
]}

Hofmann, Johann ^{[3
]}

von Eiff, Christof ^{[6
]}

Lass-Florl, Cornelia ^{[1
]}

机构：

[1] Innsbruck Med Univ, Dept Hyg Microbiol & Social Med, A-6020 Innsbruck, Austria

[2] Innsbruck Med Univ, Dept Transfus Med, A-6020 Innsbruck, Austria

[3] Innsbruck Med Univ, Div Med Biochem, A-6020 Innsbruck, Austria

[4] Univ Hosp, Dept Anesthesiol & Intens Care, Munster, Germany

[5] Univ Hosp, Dept Expt & Clin Hemostasis, Munster, Germany

[6] Univ Hosp, Dept Med Microbiol, Munster, Germany

[7] Radboud Univ Nijmegen, Med Ctr, Dept Hematol, NL-6525 ED Nijmegen, Netherlands

来源：

JOURNAL OF INFECTIOUS DISEASES | 2008年 / 198卷 / 08期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1086/591458

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Using laser scanning microscopy, we investigated whether platelets are capable of internalizing Aspergillus conidia and examined Aspergillus-platelet adherence. The influence of platelets on fungal growth was evaluated by assessing galactomannan (GM) release, hyphal elongation, and colony size. A secretion assay with [H-3]-serotonin (5-hydroxytryptamine [5-HT]) was performed. Exposure to platelets resulted in significantly decreased GM release (P <.05), hyphal elongation (P <.001), colony size, pigmentation, and 5-HT release (P <.05). A lack of antifungal effects was observed with the microfilament inhibitor cytochalasin D. Platelets attenuate the virulence of Aspergillus species in vitro on the basis of granule-dependent effects.

引用

页码：1243 / 1246

页数：4

共 15 条

[1] Thrombocytopenia in liver transplant recipients - Predictors, impact on fungal infections, and role of endogenous thrombopoietin [J].

Chang, FY ;

Singh, N ;

Gayowski, T ;

Wagener, MM ;

Mietzner, SM ;

Stout, JE ;

Marino, IR .

TRANSPLANTATION, 2000, 69 (01) :70-75

[2] Human platelets damage Aspergillus fumigatus hyphae and may supplement killing by neutrophils [J].

Christin, L ;

Wysong, DR ;

Meshulam, T ;

Hastey, R ;

Simons, ER ;

Diamond, RD .

INFECTION AND IMMUNITY, 1998, 66 (03) :1181-1189

[3] THROMBOSIS AND HEMOSTASIS [J].

COLMAN, RW ;

STEWART, G ;

BUDZYNSKI, A ;

ITTYERAH, TR ;

OLEXA, S ;

KIRBY, E ;

FUKAMI, M ;

PIPERNO, J .

NATURE, 1979, 282 (5740) :676-676

[4] Invasive aspergillosis [J].

Denning, DW .

CLINICAL INFECTIOUS DISEASES, 1998, 26 (04) :781-803

[5] INTERACTION OF HISTOPLASMA-CAPSULATUM WITH HUMAN-PLATELETS [J].

DESPREZ, RM ;

STECKLEY, S ;

STROUD, RM ;

HAWIGER, J .

JOURNAL OF INFECTIOUS DISEASES, 1980, 142 (01) :32-39

[6] The interaction of bacterial pathogens with platelets [J].

Fitzgerald, J. Ross ;

Foster, Timothy J. ;

Cox, Dermot .

NATURE REVIEWS MICROBIOLOGY, 2006, 4 (06) :445-457

[7] Antifungal properties of selective serotonin reuptake inhibitors against Aspergillus species in vitro [J].

Lass-Flörl, C ;

Dierich, MP ;

Fuchs, D ;

Semenitz, E ;

Jenewein, I ;

Ledochowski, M .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 (06) :775-779

[8]

LASSFLORL C, 2005, 45 INT C ANT AG CHEM, P97

[9] Detection of galactomannan antigenemia by enzyme immunoassay for the diagnosis of invasive aspergillosis: Variables that affect performance [J].

Marr, KA ;

Balajee, SA ;

McLaughlin, L ;

Tabouret, M ;

Bentsen, C ;

Walsh, TJ .

JOURNAL OF INFECTIOUS DISEASES, 2004, 190 (03) :641-649

[10] The postantibiotic effect of N-chlorotaurine on Staphylococcus aureus.: Application in the mouse peritonitis model [J].

Nagl, M ;

Hengster, P ;

Semenitz, E ;

Gottardi, W .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (06) :805-809

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