Charge-conversion ternary polyplex with endosome disruption moiety: A technique for efficient and safe gene delivery

被引：197

作者：

Lee, Yan ^{[1
]}

Miyata, Kanjiro ^{[3
]}

Oba, Makoto ^{[2
]}

Ishii, Takehiko

Fukushima, Shigeto ^{[1
]}

Han, Muri ^{[4
]}

Koyama, Hiroyuki ^{[2
]}

Nishiyama, Nobuhiro ^{[1
,3
]}

Kataoka, Kazunori ^{[1
,3
,4
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Ctr Dis Biol & Integrat Med, Div Clin Biotechnol,Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Dept Clin Vasc Regenerat, Tokyo 1130033, Japan

[3] Univ Tokyo, Ctr Nanobio Integrat, Tokyo 1138656, Japan

[4] Univ Tokyo, Dept Mat Engn, Tokyo 1138656, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 28期

关键词：

charge conversion; DNA; gene delivery; polymers; transfection;

D O I：

10.1002/anie.200800963

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Wrapped for special delivery: A ternary polyplex, with an endosomal disruption moiety based on the charge-conversion polymer pAsp(DET-Aco), showed negative charges for serum stability and low cytotoxicity, but the charges became positive and the endosome disruption moiety was exposed (see picture). High transfection efficiency and minimal cytotoxicity were observed with primary cells. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5163 / 5166

页数：4

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