Identification and characterization of a novel gene encoding the NBS1 protein in Pyricularia oryzae

被引：3

作者：

Narukawa-Nara, Megumi ^{[1
]}

Sasaki, Kengo ^{[1
]}

Ishii, Akira ^{[1
]}

Baba, Kouhei ^{[1
]}

Amano, Kanako ^{[1
]}

Kuroki, Misa ^{[1
]}

Saitoh, Ken-ichiro ^{[2
]}

Kamakura, Takashi ^{[1
]}

机构：

[1] Tokyo Univ Sci, Fac Sci & Technol, Dept Appl Biol Sci, Noda, Chiba 278, Japan

[2] Tokyo Univ Agr & Technol, URAC, Koganei, Tokyo, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2015年 / 79卷 / 07期

关键词：

forkhead-associated (FHA) domain; Pyricularia oryzae; Nijmegen breakage syndrome (NBS); DNA repair; DNA-DAMAGE RESPONSE; S-PHASE CHECKPOINT; SCHIZOSACCHAROMYCES-POMBE; MAGNAPORTHE-GRISEA; COMPLEX; REPAIR; REPLICATION; IRRADIATION; MECHANISM; INFECTION;

D O I：

10.1080/09168451.2015.1015951

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ascomycete Pyricularia oryzae (teleomorph: Magnaporthe oryzae) causes one of the most serious diseases known as rice blast. The Nijmegen breakage syndrome protein (NBS1) is essential for DNA repair; thus, we studied the P. oryzae NBS1 homolog (PoNBS1). A PoNBS1 null mutant exhibited high sensitivity to DNA damage-inducing agents. The mutant also exhibited the retarded hyphal growth, and induced abnormal conidial germination and shape, but showed normal appressorium formation. The phenotypes of the null mutant were complemented by introducing the cDNA of PoNBS1 driven by a TrpC promoter of Aspergillus nidulans. In addition, the null mutant similarly complemented with the PoNBS1 cDNA lacking the FHA domain that had a normal phenotype except for hyphal growth. These results suggest that PoNBS1 is involved in DNA repair and normal development in P. oryzae. Moreover, the FHA domain of PoNBS1 participates in normal hyphal growth.

引用

页码：1183 / 1190

页数：8