A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms

被引：26

作者：

Yi, Li ^{[1
]}

Wu, Ting ^{[1
]}

Luo, Wenyuan ^{[1
]}

Zhou, Wen ^{[2
]}

Wu, Jun ^{[1
]}

机构：

[1] Peking Univ, Shenzhen Hosp, Dept Neurol, Shenzhen 518036, Guangdong, Peoples R China

[2] Peking Univ, Shenzhen Hosp, Dept Radiol, Shenzhen 518036, Guangdong, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2014年 / 9卷 / 01期

关键词：

nerve regeneration; neurodegeneration; late-onset Alzheimer's disease; apolipoprotein E gene; real-time PCR; DNA sequencing; risk factor; allele; neural regeneration; TRANSGENIC MOUSE MODEL; APOE EPSILON-4 ALLELE; A-BETA; COGNITIVE IMPAIRMENT; TYPE-4; ALLELE; BUCCAL SWABS; RISK-FACTORS; GENOMIC DNA; COLLECTION; NEUROPATHOLOGY;

D O I：

10.4103/1673-5374.125332

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The apolipoprotein E gene epsilon 4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer's disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E epsilon 4 allele in neural regeneration in late-onset Alzheimer's disease cases.

引用

页码：69 / 75

页数：7

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