Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish

被引：88

作者：

Beyer, Hannes M. ^{[1
,2
,3
]}

Juillot, Samuel ^{[1
,2
,3
]}

Herbst, Kathrin ^{[4
,5
,6
]}

Samodelov, Sophia L. ^{[1
,2
,3
]}

Mueller, Konrad ^{[1
]}

Schamel, Wolfgang W. ^{[1
,2
,3
,7
]}

Roemer, Winfried ^{[1
,2
,3
]}

Schaefer, Eberhard ^{[1
]}

Nagy, Ferenc ^{[1
,8
]}

Straehle, Uwe ^{[4
,5
]}

Weber, Wilfried ^{[1
,2
,3
,9
]}

Zurbriggen, Matias D. ^{[1
,2
]}

机构：

[1] Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany

[2] Univ Freiburg, BIOSS Ctr Biol Signalling Studies, D-79104 Freiburg, Germany

[3] Univ Freiburg, SGBM Spemann Grad Sch Biol & Med SGBM, D-79104 Freiburg, Germany

[4] Karlsruhe Inst Technol, Inst Toxicol & Genet, D-76344 Eggenstein Leopoldshafen, Germany

[5] Heidelberg Univ, D-76344 Eggenstein Leopoldshafen, Germany

[6] BIF IGS BioInterfaces Int Grad Sch, D-76344 Eggenstein Leopoldshafen, Germany

[7] Univ Clin Freiburg, CCI, D-79106 Freiburg, Germany

[8] Biol Res Ctr, Inst Plant Biol, H-6726 Szeged, Hungary

[9] Univ Freiburg, ZBSA Ctr Biosyst Anal, D-79104 Freiburg, Germany

来源：

ACS SYNTHETIC BIOLOGY | 2015年 / 4卷 / 09期

基金：

欧洲研究理事会;

关键词：

light-inducible nuclear transport; optogenetics; phytochrome; mammalian synthetic biology; plant synthetic biology; SPATIOTEMPORAL CONTROL; GENE-EXPRESSION; MEDIATED INDUCTION; DEGRADATION; STABILITY;

D O I：

10.1021/acssynbio.5b00004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein trafficking in and out of the nucleus represents a key step in controlling cell fate and function. Here we report the development of a red light-inducible and far-red light-reversible synthetic system for controlling nuclear localization of proteins in mammalian cells and zebrafish. First, we synthetically reconstructed and validated the red light-dependent Arabidopsis phytochrome B nuclear import mediated by phytochrome-interacting factor 3 in a nonplant environment and support current hypotheses on the import mechanism in planta. On the basis of this principle we next regulated nuclear import and activity of target proteins by the spatiotemporal projection of light patterns. A synthetic transcription factor was translocated into the nucleus of mammalian cells and zebrafish to drive transgene expression. These data demonstrate the first in vivo application of a plant phytochrome-based optogenetic tool in vertebrates and expand the repertoire of available light-regulated molecular devices.

引用

页码：951 / 958

页数：8

共 37 条

[1] Restriction of intestinal stem cell expansion and the regenerative response by YAP [J].