Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP

被引：1

作者：

Watanabe, Leandra ^{[1
]}

de Moura, Patricia Ribeiro ^{[1
]}

Nascimento, Alessandro Silva ^{[1
]}

Colau, Didier

Dumoutier, Laure ^{[2
]}

Renauld, Jean-Christophe ^{[2
]}

Polikarpov, Igor ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil

[2] Catholic Univ Louvain, Christian Duve Inst, Expt Med Unit, B-1200 Brussels, Belgium

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2009年 / 65卷

基金：

巴西圣保罗研究基金会;

关键词：

INDUCIBLE FACTOR; INTERLEUKIN (IL)-22; CRYSTAL-STRUCTURE; HOST-DEFENSE; IL-TIF; CYTOKINE; CLONING; IL-10; IDENTIFICATION; EXPRESSION;

D O I：

10.1107/S1744309108042309

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin-22 (IL-22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL-22 was incubated with its soluble decoy receptor IL-22BP (IL-22 binding protein) and the IL-22 -IL-22BP complex was crystallized in hanging drops using the vapour-diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 angstrom resolution. The crystal belonged to the tetragonal space group P41, with unit-cell parameters a = b = 67.9, c = 172.5 angstrom, and contained two IL-22-IL- 22BP complexes per asymmetric unit.

引用

页码：102 / 104

页数：3

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