EphA2/EphrinA1 mRNA Expression and Protein Production in Adenoid Cystic Carcinoma of Salivary Gland

被引：21
作者：
Shao, Zhe [1 ,2 ,3 ]
Zhu, Fei [1 ,2 ]
Song, Kai [1 ,2 ]
Zhang, HanZhong [1 ,2 ]
Liu, Ke [1 ,2 ,3 ]
Shang, ZhengJun [1 ,2 ,3 ]
机构：
[1] State Key Lab Breeding Base Basic Sci Stomatol Hu, Wuhan, Peoples R China
[2] Minist Educ, Key Lab Oral Biomed, Wuhan, Peoples R China
[3] Wuhan Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial & Head & Neck Oncol, Wuhan 430079, Peoples R China
来源：
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY | 2013年 / 71卷 / 05期
基金：
中国国家自然科学基金;
关键词：
RECEPTOR TYROSINE KINASE; SQUAMOUS-CELL CARCINOMA; EPHA2; RECEPTOR; TUMOR ANGIOGENESIS; POOR-PROGNOSIS; CANCER; OVEREXPRESSION; SURVIVAL; BEHAVIOR; FEATURES;
D O I：
10.1016/j.joms.2012.10.026
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Purpose: EphA2/ephrinA1 is believed to play a role in tumor growth and metastasis. The purpose of the present study was to determine the presence of EphA2/ephrinA1 in mRNA and protein adenoid cystic carcinoma. Materials and Methods: mRNA and protein expression and protein product of EphA2 and ephrinA1 in adenoid cystic carcinoma was investigated using real-time reverse transcriptase polymerase chain reaction, Western blot, and immunohistochemistry. The tyrosine-phosphorylated state of EphA2 in adenoid cystic carcinoma cells was also investigated. Results: Greater expression of EphA2 and ephrinA1 proteins and mRNA was detected in adenoid cystic carcinoma tissues. EphA2/ephrinA1 staining activities in adenoid cystic carcinoma were more significant than those in normal gland tissue (P < .01). EphA2/ephrinA1 expression correlated significantly to the microvessel density (P < .01). EphA2/ephrinA1 expression and microvessel density correlated with the clinical TNM stage, perineural invasion, and vascular invasion (P < .05). In 3 histologic types of adenoid cystic carcinoma, the expression of EphA2/ephrinA1 and microvessel density was significantly greater in the solid type than in the cribriform and tubular types (P < .01). We also noted that EphA2 was present in a nontyrosine-phosphorylated state. Conclusions: The present study showed a high expression of EphA2/ephrinA1 in adenoid cystic carcinoma. EphA2/ephrinA1 can serve as a novel therapy target for adenoid cystic carcinoma. (C) 2013 American Association of Oral and Maxillofacial Surgeons J Oral Maxillofac Surg 71:869-878, 2013
引用
收藏
页码：869 / 878
页数：10
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