The two-pore domain K+ channel TRESK is expressed in dorsal root ganglion and trigeminal sensory neurons where it is a major contributor to background K+ current. TRESK acts as a break to prevent excessive sensory neuron activation and decreases in its expression or function have been involved in neuronal hyperexcitability after injury/inflammation, migraine or altered sensory perception (tingling, cooling and pungent burning sensations). All these effects have implicated this channel in nociception and mechanotransduction. To determine the role of TRESK in sensory transduction, we studied its sensitivity to changes in membrane tension (stretch) in heterologous systems, F-11 cells and trigeminal neurons. Laminar shear stress increased TRESK currents by 22-30%. An increase in membrane tension induced by cell swelling (hypotonic medium) produced a reversible elevation of TRESK currents (39.9%). In contrast, cell shrinkage (hypertonic solution) produced the opposite effect. Membrane crenators or cup-formers produced equivalent effects. In trigeminal sensory neurons, TRESK channels were mechanically stimulated by negative pressure, which led to a 1.51-fold increase in channel open probability. TRESK-like currents in trigeminal neurons were additively inhibited by arachidonic acid, acidic pH and hypertonic stimulation, conditions usually found after tissue inflammation. Our results show that TRESK is modulated by changes in cell membrane tension and/or cell volume. Several key players released during inflammation or tissue injury could modulate sensory neuron activation through small changes in membrane tension.
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Chemin, J
;
Patel, AJ
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机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Patel, AJ
;
Duprat, F
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h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Duprat, F
;
Lauritzen, I
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h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Lauritzen, I
;
Lazdunski, M
论文数: 0引用数: 0
h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Lazdunski, M
;
Honoré, E
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h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Coste, Bertrand
;
Xiao, Bailong
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Xiao, Bailong
;
Santos, Jose S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Santos, Jose S.
;
Syeda, Ruhma
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h-index: 0
机构:
Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Syeda, Ruhma
;
Grandl, Joerg
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h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Grandl, Joerg
;
Spencer, Kathryn S.
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h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Spencer, Kathryn S.
;
Kim, Sung Eun
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h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Kim, Sung Eun
;
Schmidt, Manuela
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Schmidt, Manuela
;
Mathur, Jayanti
论文数: 0引用数: 0
h-index: 0
机构:
Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Mathur, Jayanti
;
Dubin, Adrienne E.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Dubin, Adrienne E.
;
Montal, Mauricio
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h-index: 0
机构:
Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Montal, Mauricio
;
Patapoutian, Ardem
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Coste, Bertrand
;
Mathur, Jayanti
论文数: 0引用数: 0
h-index: 0
机构:
Novartis Res Fdn GNF, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Mathur, Jayanti
;
Schmidt, Manuela
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Schmidt, Manuela
;
Earley, Taryn J.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Earley, Taryn J.
;
Ranade, Sanjeev
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Ranade, Sanjeev
;
Petrus, Matt J.
论文数: 0引用数: 0
h-index: 0
机构:
Novartis Res Fdn GNF, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Petrus, Matt J.
;
Dubin, Adrienne E.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Dubin, Adrienne E.
;
Patapoutian, Ardem
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Novartis Res Fdn GNF, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Chemin, J
;
Patel, AJ
论文数: 0引用数: 0
h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Patel, AJ
;
Duprat, F
论文数: 0引用数: 0
h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Duprat, F
;
Lauritzen, I
论文数: 0引用数: 0
h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Lauritzen, I
;
Lazdunski, M
论文数: 0引用数: 0
h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
Lazdunski, M
;
Honoré, E
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h-index: 0
机构:
Int Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, FranceInt Paul Hamel, CNRS, UMR 6097, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Coste, Bertrand
;
Xiao, Bailong
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Xiao, Bailong
;
Santos, Jose S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Santos, Jose S.
;
Syeda, Ruhma
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Syeda, Ruhma
;
Grandl, Joerg
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Grandl, Joerg
;
Spencer, Kathryn S.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Spencer, Kathryn S.
;
Kim, Sung Eun
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Kim, Sung Eun
;
Schmidt, Manuela
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Schmidt, Manuela
;
Mathur, Jayanti
论文数: 0引用数: 0
h-index: 0
机构:
Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Mathur, Jayanti
;
Dubin, Adrienne E.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Dubin, Adrienne E.
;
Montal, Mauricio
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Montal, Mauricio
;
Patapoutian, Ardem
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, Dorris Neurosci Ctr, La Jolla, CA 92037 USA
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Coste, Bertrand
;
Mathur, Jayanti
论文数: 0引用数: 0
h-index: 0
机构:
Novartis Res Fdn GNF, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Mathur, Jayanti
;
Schmidt, Manuela
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Schmidt, Manuela
;
Earley, Taryn J.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Earley, Taryn J.
;
Ranade, Sanjeev
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Ranade, Sanjeev
;
Petrus, Matt J.
论文数: 0引用数: 0
h-index: 0
机构:
Novartis Res Fdn GNF, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Petrus, Matt J.
;
Dubin, Adrienne E.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Dubin, Adrienne E.
;
Patapoutian, Ardem
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
Novartis Res Fdn GNF, Genom Inst, San Diego, CA 92121 USAScripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA