Measuring acoustic energy density in microchannel acoustophoresis using a simple and rapid light-intensity method

被引：48

作者：

Barnkob, Rune ^{[1
]}

Iranmanesh, Ida ^{[2
]}

Wiklund, Martin ^{[2
]}

Bruus, Henrik ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Micro & Nanotechnol, DK-2800 Lyngby, Denmark

[2] AlbaNova Univ Ctr, Royal Inst Technol, Dept Appl Phys, SE-10691 Stockholm, Sweden

来源：

LAB ON A CHIP | 2012年 / 12卷 / 13期

基金：

瑞典研究理事会; 欧盟第七框架计划;

关键词：

ULTRASONIC STANDING-WAVE; CHIP; CELL; MANIPULATION; SUSPENSIONS; TRAP;

D O I：

10.1039/c2lc40120g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a simple and rapid method for measuring the acoustic energy density in microchannel acoustophoresis based on light-intensity measurements of a suspension of particles. The method relies on the assumption that each particle in the suspension undergoes single-particle acoustophoresis. It is validated by the single-particle tracking method, and we show by proper re-scaling that the re-scaled light intensity plotted versus re-scaled time falls on a universal curve. The method allows for analysis of moderate-resolution images in the concentration range encountered in typical experiments, and it is an attractive alternative to particle tracking and particle image velocimetry for quantifying acoustophoretic performance in microchannels.

引用

页码：2337 / 2344

页数：8

共 25 条

[11]

Happel J., 1983, Mechanics of Fluids and Transport Processes

[12] On-chip measurements of cell compressibility via acoustic radiation [J].

Hartono, Deny ;

Liu, Yang ;

Tan, Pei Lin ;

Then, Xin Yi Sherlene ;

Yung, Lin-Yue Lanry ;

Lim, Kian-Meng .

LAB ON A CHIP, 2011, 11 (23) :4072-4080

[13] Proliferation and viability of adherent cells manipulated by standing-wave ultrasound in a microfluidic chip [J].

Hultstrom, J. ;

Manneberg, O. ;

Dopf, K. ;

Hertz, H. M. ;

Brismar, H. ;

Wiklund, M. .

ULTRASOUND IN MEDICINE AND BIOLOGY, 2007, 33 (01) :145-151

[14] Acoustophoresis in shallow microchannels [J].

Koklu, Mehti ;

Sabuncu, Ahmet Can ;

Beskok, Ali .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2010, 351 (02) :407-414

[15] Fractionation of cell mixtures using acoustic and laminar flow fields [J].

Kumar, M ;

Feke, DL ;

Belovich, JM .

BIOTECHNOLOGY AND BIOENGINEERING, 2005, 89 (02) :129-137

[16]

Limaye MS, 1998, J APPL MICROBIOL, V84, P1035

[17] Flow-free transport of cells in microchannels by frequency-modulated ultrasound [J].

Manneberg, O. ;

Vanherberghen, B. ;

Onfelt, B. ;

Wiklund, M. .

LAB ON A CHIP, 2009, 9 (06) :833-837

[18] Spore and micro-particle capture on an immunosensor surface in an ultrasound standing wave system [J].

Martin, SP ;

Townsend, RJ ;

Kuznetsova, LA ;

Borthwick, KAJ ;

Hill, M ;

McDonnell, MB ;

Coakley, WT .

BIOSENSORS & BIOELECTRONICS, 2005, 21 (05) :758-767

[19] Microfluidic capturing-dynamics of paramagnetic bead suspensions [J].

Mikkelsen, C ;

Bruus, H .

LAB ON A CHIP, 2005, 5 (11) :1293-1297

[20] THE CLINICAL IMPORTANCE OF ERYTHROCYTE DEFORMABILITY, A HEMORRHEOLOGICAL PARAMETER [J].

MOKKEN, FC ;

KEDARIA, M ;

HENNY, CP ;

HARDEMAN, MR ;

GELB, AW .

ANNALS OF HEMATOLOGY, 1992, 64 (03) :113-122

← 1 2 3 →